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- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/09%3A_Regulation_of_Gene_Expression/9.11%3A_Cell-specific_gene_expressionThis page discusses gene expression study methods, particularly using transgenic techniques in Drosophila with the even-skipped gene and beta-galactosidase for visualization. It highlights green fluor...This page discusses gene expression study methods, particularly using transgenic techniques in Drosophila with the even-skipped gene and beta-galactosidase for visualization. It highlights green fluorescent protein (GFP) for real-time monitoring in living cells and advancements in DNA chip technology for analyzing thousands of genes simultaneously.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/06%3A_Gene_Expression/6.06%3A_Expressed_Sequence_TagsThis page discusses vertebrate DNA, emphasizing that only a small percentage encodes proteins, while much consists of non-coding regions. Identifying genes is complex, even with complete genomic data....This page discusses vertebrate DNA, emphasizing that only a small percentage encodes proteins, while much consists of non-coding regions. Identifying genes is complex, even with complete genomic data. Transcriptome analysis, which examines mRNA from various cell types, aids in gene identification. Expressed Sequence Tags (ESTs) are used to determine gene activity by isolating mRNA, converting it to cDNA, and sequencing it for genomic matches.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Fundamentals_of_Biochemistry_(Jakubowski_and_Flatt)/01%3A_Unit_I-_Structure_and_Catalysis/09%3A_Investigating_DNA/9.01%3A_DNA_Isolation_Sequencing_and_SynthesisThe document provides a comprehensive overview of DNA technologies, focusing on DNA extraction, sequencing, and synthesis. It distinguishes between genomic and complementary DNA, describing their stru...The document provides a comprehensive overview of DNA technologies, focusing on DNA extraction, sequencing, and synthesis. It distinguishes between genomic and complementary DNA, describing their structures and roles. DNA sequencing methods, including Sanger sequencing and Next-Generation Sequencing (NGS), are explored, highlighting advancements and their applications in genomics.
