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- https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Introduction_to_Biotechnology/01%3A_Techniques/1.20%3A_Good_Manufacturing_Practices_(GMPs)Construct a flow diagram of the following overview of the training and production of your component for the snack product today. The completed ASC Training Document must be turned in to the supervisor...Construct a flow diagram of the following overview of the training and production of your component for the snack product today. The completed ASC Training Document must be turned in to the supervisor (instructor) to receive material to start the production run. It enables a team to brainstorm and categorize all the potential problems that could be the cause of a problem so they can be systematically eliminated until the real cause or causes is/are identified.
- https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Introduction_to_Biotechnology/00%3A_Front_Matter/02%3A_InfoPageThe LibreTexts libraries are Powered by MindTouch ® and are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the Californ...The LibreTexts libraries are Powered by MindTouch ® and are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot.
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.02%3A_Lab_Manual-_Introduction_to_Biotechnology/2.2.11%3A_Restriction_Digest_with_Gel_ElectrophorisisWhen the agarose solution has cooled to the point that you can safely touch the bottom of the flask (~60°C; about five minutes), pour agarose solution into each casting tray, so that the solution cove...When the agarose solution has cooled to the point that you can safely touch the bottom of the flask (~60°C; about five minutes), pour agarose solution into each casting tray, so that the solution covers about 2 mm of each comb. The solidified agarose gel matrix will have pores of various sizes (similar to a sponge), so the size, shape and charge of the molecules can affect the rate of travel through the agarose gel.
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.02%3A_Lab_Manual-_Introduction_to_Biotechnology/2.2.02%3A_Metrics_and_MeasurementsIf a student uses a thermometer calibrated to the nearest degree to measure the temperature of a beaker of water and a second student uses a thermometer that is calibrated to the nearest ten degrees f...If a student uses a thermometer calibrated to the nearest degree to measure the temperature of a beaker of water and a second student uses a thermometer that is calibrated to the nearest ten degrees for the same exercise, the reproducibility or precision of measurements will be greater for the student using the thermometer calibrated to the nearest degree.
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.02%3A_Lab_Manual-_Introduction_to_Biotechnology/2.2.12%3A_TransformationThe ampR gene confers resistance to the antibiotic ampicillin. (Biotechnologists call these genes selectable markers because only bacteria having the gene will survive in the presence of an antibiotic...The ampR gene confers resistance to the antibiotic ampicillin. (Biotechnologists call these genes selectable markers because only bacteria having the gene will survive in the presence of an antibiotic.) If the inducer is present in the bacteria, the promoter will be “turned on” so RNA polymerase can transcribe the gene of interest.
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.02%3A_Lab_Manual-_Introduction_to_Biotechnology/2.2.14%3A_SDS-PAGEThe top portion is referred to as the “stacking gel” and the lower portion is termed the “running gel” or “separating gel”. The purpose of the stacking gel is to concentrate all of the different sized...The top portion is referred to as the “stacking gel” and the lower portion is termed the “running gel” or “separating gel”. The purpose of the stacking gel is to concentrate all of the different sized proteins into a compact horizontal zone by sandwiching them between a gradient of glycine molecules above and chloride ions below.
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.02%3A_Lab_Manual-_Introduction_to_Biotechnology
- https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Introduction_to_Biotechnology/01%3A_Techniques/1.14%3A_Column_ChromatographyFinally, the elution buffer, which has a low buffer concentration, causes the protein to begin to refold to hide the hydrophobic amino acids which releases the protein from the resin coated beads in t...Finally, the elution buffer, which has a low buffer concentration, causes the protein to begin to refold to hide the hydrophobic amino acids which releases the protein from the resin coated beads in the column. The reason the cells can be induced to produce protein is that within the plasmid, in front of the gene for gfp, there is a special sequence of DNA that will respond if a chemical is placed in the media.
- https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Introduction_to_Biotechnology/00%3A_Front_Matter/03%3A_AttributionsThe faculty of the Orange County Biotechnology Education Collaborative (otherwise known as OCBiotechEducation.org), authored or adapted the laboratories contained in this manual for the purpose of con...The faculty of the Orange County Biotechnology Education Collaborative (otherwise known as OCBiotechEducation.org), authored or adapted the laboratories contained in this manual for the purpose of constructing an Open Educational Resource (OER) for students beginning studies in the field of Biotechnology.
- https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Introduction_to_Biotechnology/01%3A_Techniques/1.10%3A_Enzyme_FunctionA change in pH can alter the bonds of the 3-dimensional shape of an enzyme and cause the enzyme to change shape, which may slow or prohibit binding of the substrate to the active site. If the color of...A change in pH can alter the bonds of the 3-dimensional shape of an enzyme and cause the enzyme to change shape, which may slow or prohibit binding of the substrate to the active site. If the color of the I 2 KI continues to change to a darker color after a total of 8 minutes of testing, stop testing that reaction mixture, record 480 seconds as the time in the data table, and proceed with step 9.
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.02%3A_Lab_Manual-_Introduction_to_Biotechnology/2.2.16%3A_ELISAIn the ELISA conducted for this lab, the antigen (from HIV virus) is adsorbed to the surface of the plastic wells (on the 8-well strip or 96-well plate). In summary, for patients with HIV, the antibod...In the ELISA conducted for this lab, the antigen (from HIV virus) is adsorbed to the surface of the plastic wells (on the 8-well strip or 96-well plate). In summary, for patients with HIV, the antibodies in their blood bind to the HIV antigen, the secondary antibody will bind to the human antibodies, and the enzyme will produce a colored product that is easy to visualize.
