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- https://bio.libretexts.org/Bookshelves/Microbiology/Introductory_Bacteriology_Lab_Manual_(Turnbull)/00%3A_Front_Matter/02%3A_InfoPageThe LibreTexts libraries are Powered by NICE CXOne and are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the Californi...The LibreTexts libraries are Powered by NICE CXOne and are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot.
- https://bio.libretexts.org/Bookshelves/Microbiology/Introductory_Bacteriology_Lab_Manual_(Turnbull)/zz%3A_Back_Matter/21%3A_Detailed_Licensing
- https://bio.libretexts.org/Bookshelves/Microbiology/Introductory_Bacteriology_Lab_Manual_(Turnbull)This is an introductory bacteriology lab manual used in the Chemical Laboratory Technology and Environmental Technology advanced diploma programs at Fanshawe College. These labs provide students with ...This is an introductory bacteriology lab manual used in the Chemical Laboratory Technology and Environmental Technology advanced diploma programs at Fanshawe College. These labs provide students with an opportunity to demonstrate lab skills. Students will isolate an organism from the environment, then, using controlled experiments, determine the identity of the organism using morphology, physiology and molecular methods.
- https://bio.libretexts.org/Bookshelves/Microbiology/Introductory_Bacteriology_Lab_Manual_(Turnbull)/00%3A_Front_Matter/01%3A_TitlePageIntroductory-Bacteriology-Lab-Manual-1704982056.epub
- https://bio.libretexts.org/Bookshelves/Microbiology/Introductory_Bacteriology_Lab_Manual_(Turnbull)/01%3A_Labs/1.02%3A_Basic_TechniquesThis is to melt the agar, otherwise the agar settles to the bottom of the flask and you would have an unequal distribution of agar in your tubes after dispensing the media (some tubes would have littl...This is to melt the agar, otherwise the agar settles to the bottom of the flask and you would have an unequal distribution of agar in your tubes after dispensing the media (some tubes would have little agar and be liquid and some, with too much agar, would be solid). The volume adjustment knob can be the same as the push button, or it might be a black dial (called a thumbwheel) at the top of the pipette body.
- https://bio.libretexts.org/Bookshelves/Microbiology/Introductory_Bacteriology_Lab_Manual_(Turnbull)/01%3A_Labs/1.05%3A_Gram_Stain_and_Potassium_Hydroxide_String_TestSterilize the loop then use it to pick up a small amount of bacteria off the plate. (If too many bacteria are placed on the smear, it will be too thick to see through under the microscope.) The flocs ...Sterilize the loop then use it to pick up a small amount of bacteria off the plate. (If too many bacteria are placed on the smear, it will be too thick to see through under the microscope.) The flocs may settle on the bottom of the tube and can be differentiated from precipitation by the presence of chunks. Gently wash the slide with distilled water by holding the slide on an angle and allowing the water to wash over the smears.
- https://bio.libretexts.org/Bookshelves/Microbiology/Introductory_Bacteriology_Lab_Manual_(Turnbull)/00%3A_Front_Matter/04%3A_LicensingA detailed breakdown of this resource's licensing can be found in Back Matter/Detailed Licensing.
- https://bio.libretexts.org/Bookshelves/Microbiology/Introductory_Bacteriology_Lab_Manual_(Turnbull)/01%3A_Labs
- https://bio.libretexts.org/Bookshelves/Microbiology/Introductory_Bacteriology_Lab_Manual_(Turnbull)/01%3A_Labs/1.10%3A_Food_LabThe bacteria on the food sample may be stressed and not in their optimal conditions, thus, if we are trying to count them in the lab, we will treat them as gently as possible to prevent killing these ...The bacteria on the food sample may be stressed and not in their optimal conditions, thus, if we are trying to count them in the lab, we will treat them as gently as possible to prevent killing these already stressed bacteria. The presence and number of spoilage bacteria depends on factors related to the food itself, called intrinsic factors, and factors related to the handling and storage of the food, called extrinsic factors.
- https://bio.libretexts.org/Bookshelves/Microbiology/Introductory_Bacteriology_Lab_Manual_(Turnbull)/01%3A_Labs/1.09%3A_Bacteriological_Analysis_of_WaterIn Ontario, drinking water is regulated provincially by the Ministry of the Environment and Climate Change (MoECC), while bottled water is regulated federally by the Food and Drug Act. The number of p...In Ontario, drinking water is regulated provincially by the Ministry of the Environment and Climate Change (MoECC), while bottled water is regulated federally by the Food and Drug Act. The number of positive tubes is recorded and this number is used to refer to a table, which gives a probable range of CFU/100 ml. Set up the vacuum pump and filter apparatus, flame the alcohol off the forceps then aseptically place the membrane filter on the apparatus.
- https://bio.libretexts.org/Bookshelves/Microbiology/Introductory_Bacteriology_Lab_Manual_(Turnbull)/01%3A_Labs/1.07%3A_Unknown_Molecular_Lab_Part_IAt the end of the procedure, pure DNA remains in the solution while other cellular components are bound to a particle (called Chelex) and form a precipitate at the bottom of the tube. If the 16S rDNA ...At the end of the procedure, pure DNA remains in the solution while other cellular components are bound to a particle (called Chelex) and form a precipitate at the bottom of the tube. If the 16S rDNA gene wasn’t amplified, it would exist at the same concentration as the rest of the genes on the genome and it would be difficult to determine the sequence of this gene.
