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- https://bio.libretexts.org/Bookshelves/Biochemistry/Fundamentals_of_Biochemistry_(Jakubowski_and_Flatt)/01%3A_Unit_I-_Structure_and_Catalysis/05%3A_Protein_Function/5.02%3A_Techniques_to_Measure_BindingIt is often essential to determine the KD for a ML complex since given that number and the concentrations of M and L in the system, we can then predict if M is bound under physiological conditions. Ag...It is often essential to determine the KD for a ML complex since given that number and the concentrations of M and L in the system, we can then predict if M is bound under physiological conditions. Again, this is important since whether M is bound or free will govern its activity. To determine KD, you need to determine ML and L at equilibrium. How can we differentiate free from bound ligand? The following techniques allow such a differentiation.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Fundamentals_of_Biochemistry_(Jakubowski_and_Flatt)/01%3A_Unit_I-_Structure_and_Catalysis/03%3A_Amino_Acids_Peptides_and_Proteins/3.3%3A_Protein_PurificationThe page describes the key concepts and steps involved in protein purification and characterizes different techniques used in the purification process. It outlines various chromatographic methods such...The page describes the key concepts and steps involved in protein purification and characterizes different techniques used in the purification process. It outlines various chromatographic methods such as ion exchange, size-exclusion, hydrophobic interaction, and affinity chromatography. Additionally, it explains electrophoresis methods like SDS-PAGE and 2D electrophoresis.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Fundamentals_of_Biochemistry_(Jakubowski_and_Flatt)/01%3A_Unit_I-_Structure_and_Catalysis/03%3A_Amino_Acids_Peptides_and_Proteins/3.5%3A_Extension_-_Antibodies_in_Quantitation_and_In_Vivo_DetectionThe page details the fundamentals of antibodies in biochemistry, focusing on their structure, function, and the techniques used to utilize them. Key areas include understanding antibody-antigen intera...The page details the fundamentals of antibodies in biochemistry, focusing on their structure, function, and the techniques used to utilize them. Key areas include understanding antibody-antigen interactions, exploring quantitation methods like ELISA, and discussing techniques like immunohistochemistry (IHC) used for detecting proteins in vivo.
