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- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/11%3A_Genomics/11.08%3A_Gel_BlottingThis page discusses gel blotting, a method to visualize specific macromolecules such as proteins and DNA/RNA through electrophoresis and transfer onto a nitrocellulose filter. The Southern Blot, creat...This page discusses gel blotting, a method to visualize specific macromolecules such as proteins and DNA/RNA through electrophoresis and transfer onto a nitrocellulose filter. The Southern Blot, created by E. M. Southern, identifies DNA fragments using radiolabeled probes. Analogous techniques for RNA and proteins are known as Northern and Western blots, employing similar procedures for detection.
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Genetics/12%3A_Techniques_of_Molecular_Genetics/12.07%3A__DNA_Analysis-_Blotting_and_HybridizationBands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such a...Bands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such as after restriction digestion of chromosomal (genomic) DNA, there will be a large number of variable size fragments in the digest and it will appear as a continuous smear of DNA, rather than distinct bands.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Book%3A_Biochemistry_Free_For_All_(Ahern_Rajagopal_and_Tan)/08%3A_Basic_Techniques/8.04%3A_Detection_identification_and_quantitation_of_specific_nucleic_acids_and_proteinsOne way to detect the presence of a particular nucleic acid or protein is dependent on transferring the separated molecules from the gels onto a membrane made of nitrocellulose or nylon to create a “b...One way to detect the presence of a particular nucleic acid or protein is dependent on transferring the separated molecules from the gels onto a membrane made of nitrocellulose or nylon to create a “blot” and probing for the molecule(s) of interest using reagents that specifically bind to those molecules. The next section will discuss how this can be done for nucleic acids as well as for proteins.
- https://bio.libretexts.org/Bookshelves/Genetics/Online_Open_Genetics_(Nickle_and_Barrette-Ng)/08%3A_Techniques_of_Molecular_Genetics/8.07%3A__DNA_Analysis-_Blotting_and_HybridizationBands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such a...Bands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such as after restriction digestion of chromosomal (genomic) DNA, there will be a large number of variable size fragments in the digest and it will appear as a continuous smear of DNA, rather than distinct bands.
- https://bio.libretexts.org/Courses/Ohio_State_University/Ohio_State_University_SP22%3A_Molecular_Genetics_4606_(Chamberlin)/13%3A_Detecting_Genes_and_Gene_Products/13.01%3A__DNA_Analysis-_Blotting_and_HybridizationBands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such a...Bands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such as after restriction digestion of chromosomal (genomic) DNA, there will be a large number of variable size fragments in the digest and it will appear as a continuous smear of DNA, rather than distinct bands.
