Typical sites of methylation include the N 6 position of adenine, the N 4 position of cytosine, or the C 5 position of cytosine. The dye is usually incorporated into the gel and running buffer, or con...Typical sites of methylation include the N 6 position of adenine, the N 4 position of cytosine, or the C 5 position of cytosine. The dye is usually incorporated into the gel and running buffer, or conversely, the gel is stained after running by soaking in a solution of ethidium bromide (0.5 ug/ml for 30 min). Any changes in the DNA sequence of a gene can result in the elimination of particular restriction sites, and conversely, create new ones:
