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- https://bio.libretexts.org/Courses/North_Central_State_College/BIOL_1550%3A_Microbiology_(2025)/02%3A_Microscopes/2.04%3A_Staining_Microscopic_SpecimensIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
- https://bio.libretexts.org/Courses/Portland_Community_College/Cascade_Microbiology/02%3A_How_We_See_the_Invisible_World/2.4%3A_Staining_Microscopic_SpecimensIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
- https://bio.libretexts.org/Courses/West_Los_Angeles_College/Biotechnology/13%3A_Biotechnology_Lab_Protocols/13.15%3A_Bacterial_Identification_MethodsThis page outlines some common bacterial staining protocols, including a simple stain with methylene blue and the differential staining protocols, Gram staining, acid-fast staining, and endospore stai...This page outlines some common bacterial staining protocols, including a simple stain with methylene blue and the differential staining protocols, Gram staining, acid-fast staining, and endospore staining. In addition, protocols for preparing bacterial smears and plates are given.
- https://bio.libretexts.org/Courses/Mansfield_University_of_Pennsylvania/BSC_3271%3A_Microbiology_for_Health_Sciences_Sp21_(Kagle)/01%3A_Introduction/1.03%3A_How_We_See_the_Invisible_World/1.3.04%3A_Staining_Microscopic_SpecimensIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
- https://bio.libretexts.org/Workbench/Clinical_Microbiology_Lab_Manual/06%3A_Acid-Fast_Spores_and_Capsule_StainsAcid-fast stain is a differential stain used to identify acid-fast organisms such as members of the genus Mycobacterium. Acid-fast organisms are characterized by wax-like, nearly impermeable cell wall...Acid-fast stain is a differential stain used to identify acid-fast organisms such as members of the genus Mycobacterium. Acid-fast organisms are characterized by wax-like, nearly impermeable cell walls; they contain mycolic acid and large amounts of fatty acids, waxes, and complex lipids.
- https://bio.libretexts.org/Bookshelves/Microbiology/Microbiology_(OpenStax)/02%3A_How_We_See_the_Invisible_World/2.04%3A_Staining_Microscopic_SpecimensIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
- https://bio.libretexts.org/Bookshelves/Microbiology/Microbiology_Laboratory_Manual_(Miller)/01%3A_Labs/1.15%3A_Acid-Fast_and_Endospore_StainingAdd carbol fuchsin (primary stain) on top of the paper to saturate: leave on for 5-7 minutes and place over a steam bath in the hood. Acid-fast organisms retain the primary stain and will appear brigh...Add carbol fuchsin (primary stain) on top of the paper to saturate: leave on for 5-7 minutes and place over a steam bath in the hood. Acid-fast organisms retain the primary stain and will appear bright red: non acid-fast organisms are decolorized with acid-alcohol and pick up the methylene blue counterstain. For this reason they are difficult to eliminate from the environment with standard methods of disinfection, and they are difficult to treat in the case of an infection.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Book%3A_General_Microbiology_Lab_Manual_(Pakpour_and_Horgan)/Lab_04%3A_Acid-Fast_Spores_and_Capsule_StainsAcid-fast stain is a differential stain used to identify acid-fast organisms such as members of the genus Mycobacterium. Acid-fast organisms are characterized by wax-like, nearly impermeable cell wall...Acid-fast stain is a differential stain used to identify acid-fast organisms such as members of the genus Mycobacterium. Acid-fast organisms are characterized by wax-like, nearly impermeable cell walls; they contain mycolic acid and large amounts of fatty acids, waxes, and complex lipids.
- https://bio.libretexts.org/Courses/Manchester_Community_College_(MCC)/Remix_of_Openstax%3AMicrobiology_by_Parker_Schneegurt_et_al/03%3A_Microscope_and_the_Cell/3.02%3A_Staining_Microscopic_SpecimensIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Microbiology_(Liu_et_al.)/02%3A_Microscopes/2.07%3A_Sample_Preparation_for_Microscopic_StainingIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/18%3A_Spore_StainThat is why there does not need to be a decolorizer in this stain: it is based on the binding of the malachite green and the permeability of the spore vs. The heating of the bacteria will make the spo...That is why there does not need to be a decolorizer in this stain: it is based on the binding of the malachite green and the permeability of the spore vs. The heating of the bacteria will make the spore wall more permeable to the malachite green, and it then attaches to the peptidoglycan. Wash really WELL with water and move the slide and wire rack from the boiling water to the regular stain tray to finish up the last step in the procedure.
