Place the loop with the bacteria into the slant tube, all the way down to the bottom of the slant. You can do this by 1) sticking the hot loop in the agar at the edge of the agar away from the bacteri...Place the loop with the bacteria into the slant tube, all the way down to the bottom of the slant. You can do this by 1) sticking the hot loop in the agar at the edge of the agar away from the bacteria, or 2) just holding the loop for a few seconds while it cools. Why streak from the bottom of the agar slant medium up to the top in a straight line, rather than a back and forth wavy inoculation from side-to-side on the slant?
