6.1: Required Materials

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Page ID: 152226

Jonathon Van Gray
Ohio State University

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Cultures Needed

TSB Cultures
- E. coli
- S. epidermidis
- S. aureus
- P. aeruginosa
- P. vulgaris
- Lactobacillus acidophilus
- Alcaligenes faecalis

Environmental Effects on Growth: pH

4 tubes: TSB pH 4.0
4 tubes: TSB pH 6.0
4 tubes: TSB pH 8.0
4 tubes: TSB pH 10.0

Environmental Effects on Growth: Osmotic Pressure

1 TSA plate of each of 2%, 5%, and 10% Sodium Chloride $NaCl " id="MathJax-Element-96-Frame" role="presentation" style="position:relative;" tabindex="0"> NaCl$

Mannitol Salt Agar

1 Mannitol Salt Agar plate

EMB Agar

3 EMB plates

SIM Deep Tests

3 SIM Deep tubes

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Margin Size

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TSB Cultures

E. coli

S. epidermidis

S. aureus

P. aeruginosa

P. vulgaris