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2.1: Required Materials

Last updated

Mar 21, 2025
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- 2: Lab 2
- 2.2: Aseptic Technique

Jonathon Van Gray
Ohio State University

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Cultures needed

TSB tubes of
- E. coli
- Serratia marcescens
- E. coli and S. marcescens mix
- Staphylococcus epidermidis
- Bacillus subtilis
- Enterobacter aerogenes
- B. subtilis (endo) – Grow at 37 C for 72 h

Aseptic Technique

TSB tubes
TSA Slant tubes
TSA Deep tubes
TSA Plates

Plating on Petri Plates for Isolation

TSA Plates
Simple Stain
Slides
Bibulous paper
Methylene blue

Gram Stain

Slides
Bibulous paper
Methylene blue
Crystal violet
Iodine
Alcohol
Safranin

Endospore Stain

Malachite green
Beakers
Wire rack for beakers
Hot plate
Paper towel
Safranin

Capsule Stain

Acid-fast Stain

Introduction to Micropipetting

Micropipettes
Micropipette tips

Aseptic Technique

TSB tubes

TSA Slant tubes

TSA Deep tubes

TSA Plates

Plating on Petri Plates for Isolation

TSA Plates

Simple Stain

Slides

Bibulous paper

Methylene blue

Gram Stain

Slides

Bibulous paper

Methylene blue

Crystal violet

Iodine

Alcohol

Safranin

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