5.5: Review Questions

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1. Why do we report bacterial counts as CFUs/mL?

2. A viable plate count was performed on a river sample. Using the table below, how many bacteria were present in the original river sample?

Dilution	Amount plated	Number of colonies counted
10^-4	1.0mL	310
10^-5	1.0mL	28
10^-6	1.0mL	7

2. How would you prepare a series of dilutions to get a final dilution of 10^-10? outline each step.

3. When determining the number of bacteria from a sample using a viable plant count, why do you often have to do a 'serial' dilution of the sample first? Why don't you just plate out 1ml or 0.1ml of the sample on a spread plate?

4. Why is it called a "viable" plate count? what does that mean?

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