14: Materials List

Week 1 – Lab Safety (Will be required throughout the semester)

• Gloves (XS, S, M, L, and XL)
• Disinfectants
• Lab coat

Week 2 – Basic Measuring Techniques

• Measuring scale or analytical scale
• Weigh boats (Carolina, 702332)
• Weigh papers (Thomas Scientific, 1229D27)
• Scoopula (Carolina, 702851)
• 50mL beaker (Carolina, 717921)
• 250mL graduate cylinders (Carolina, 721604)
• 25mL Serological pipette (USA Scientific, 1072-5410)
• 25mL Pipet pump (Carolina, 736864)
• Standard mass
• NaCl (Flinn Scientific, S0064)

Week 3 – Micro pipetting and Calibration.

• Same as Week 2.
• Micropipettes sets.
• Micropipette tips
• 1.5mL-microcentrifuge tube (Carolina, #215244)
• 50mL-High Speed conical tubes (Fisher Scientific, #352070)
• 15mL-centrifuge tube (Carolina, # 215085)
• 50mL Conical centrifuge tubes (Fisher Scientific, 352070)
• Four colored waters (Carolina, # 898030)
  • Have water stock in 500mL beaker (Carolina, 717924)

Week 4 – Dilutions and Spectrophotometer

• Same as Week 3
• 70% Ethanol spray (Flinn Scientific, E0013)
• Spectrophotometry
• Cuvette holders (Grainger, 12C181)
• Uvettes (Fisher Scientific, E4099100008)
• 500mL of 0.5M of Copper (II) Sulfate (Flinn Scientific, C0108)
• 1M Sodium acetate (Flinn Scientific, #S0038)
• 1M Acetic Acid (Flinn Scientific, #A0187)

Week 5 – pH Calibration and buffer measurements

• Same as Week 3.
• Magnetic Stir (Carolina, #701091)
• Hot Plate Stirrer Machine (Carolina, #701012)
• Stir Bar retriever (Carolina, #701089)
• pH Meter (Carolina, #186032)
• 6M Hydrochloric Acids (HCl) (Flinn Scientific, #H0033)
• 6M Sodium Hydroxide (NaOH) (Flinn Scientific, #S0242)
• pH paper (Carolina, 894720)
• pH Calibration buffers (Make sure they are NEW)
• pH Electrode storage solution (Carolina, # 185763)
• pH Electrode Cleaning solution (Carolina, # 185764)

Week 6 to Week 15 – Advance Buffer Preparations

• Same as Week 2.
• Same as Week 3.
• 125mL Erlenmeyer Flask (Carolina, #721149A)
• Tryptone (Carolina, #216741)
• Yeast extract (Carolina, #216746)
• NaCl (Flinn Scientific, #S0064)
• Aluminum foil (Carolina, #974844)
• Luria Broth Agar Base (Carolina, 216701)
• Petri dish (Carolina, # 741251)
• Calcium Chloride (Carolina, 851840)
• Tris (Flinn Scientific, #FB2393)
• Boric acid (Flinn Scientific, #B0081)
• EDTA (Gold Biotechnology, #E-210)
• Agarose
• MiniONE agarose gel electrophoresis kit

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Proper handling and disposal of hazardous materials and chemicals are essential to ensure laboratory safety and environmental protection. Below are guidelines for the reagents and supplies listed in your lab activities:

General Safety Measures:

1. Personal Protective Equipment (PPE): Always wear appropriate PPE, including gloves, lab coats, and safety goggles, when handling chemicals.
2. Ventilation: Work in well-ventilated areas, preferably under a fume hood, to minimize inhalation risks.
3. Training: Ensure all personnel are trained in chemical safety and emergency procedures.

Handling and Disposal Guidelines:

1. Gloves (XS, S, M, L, XL):
   • Handling: Use gloves suitable for the chemicals being handled.
   • Disposal: Dispose of used gloves in designated hazardous waste containers.
2. Disinfectants:
   • Handling: Follow manufacturer instructions for dilution and application.
   • Disposal: Dispose of unused disinfectants according to institutional guidelines, typically as hazardous waste.
3. Lab Coats:
   • Handling: Wear lab coats to protect against chemical spills.
   • Disposal: If contaminated with hazardous materials, launder separately or dispose of as hazardous waste.
4. Measuring Scale or Analytical Scale:
   • Handling: Calibrate regularly and clean after each use.
5. Weigh Boats and Weigh Papers:
   • Handling: Use for weighing chemicals to prevent contamination.
   • Disposal: Dispose of contaminated weigh boats and papers in hazardous waste containers.
6. Scoopula:
   • Handling: Use for transferring solid chemicals.
   • Disposal: Clean thoroughly after use
7. Beakers and Graduated Cylinders:
   • Handling: Use for mixing and measuring liquids.
   • Disposal: Clean with appropriate solvents; dispose of cleaning solutions as hazardous waste if they contain hazardous residues.
8. Serological Pipettes and Pipet Pumps:
   • Handling: Use for transferring liquids; ensure proper technique to avoid contamination.
   • Disposal: Dispose of used pipette tips in designated sharps containers; clean and reuse pipet pumps.
9. Standard Mass:
   • Handling: Use for calibration; handle with care to maintain accuracy.
   • Disposal: If damaged or contaminated, consult with a supervisor.
10. Sodium Chloride (NaCl):
    • Handling: Use as directed; avoid inhalation of dust.
    • Disposal: Dispose of excess NaCl in regular waste unless contaminated.
11. Micropipettes and Tips:
    • Handling: Use for precise liquid measurements; avoid contamination.
    • Disposal: Dispose of contaminated tips in biohazard waste containers; clean and calibrate micropipettes regularly.
12. Centrifuge Tubes (1.5mL, 15mL, 50mL):
    • Handling: Use for sample separation; ensure proper sealing.
    • Disposal: Dispose of used tubes in biohazard waste containers if contaminated.
13. Colored Waters:
    • Handling: Use as tracers or standards; handle according to chemical properties.
    • Disposal: Dispose of in the sink.
14. 70% Ethanol Spray:
    • Handling: Use for surface disinfection; avoid inhalation and open flames.
    • Disposal: Dispose of unused ethanol in designated hazardous waste containers.
15. Spectrophotometry Equipment:
    • Handling: Use for measuring light absorption; handle with care to avoid damage.
    • Disposal: Follow manufacturer's guidelines for maintenance.
16. Cuvette Holders and Uvettes:
    • Handling: Use for holding samples in spectrophotometry; clean after each use.
    • Disposal: Dispose of broken or contaminated cuvettes in waste containers.
17. Copper (II) Sulfate (0.5M):
    • Handling: Use with caution; avoid inhalation and skin contact.
    • Disposal: Dispose of excess solution in designated hazardous waste containers.
18. Sodium Acetate (1M) and Acetic Acid (1M):
    • Handling: Use as buffers; handle with care to avoid spills.
    • Disposal: Dispose of according to institutional guidelines; typically as hazardous waste.
19. Magnetic Stirrer and Hot Plate Stirrer Machine:
    • Handling: Always ensure that these devices are turned off when not in use. Handle carefully to avoid breakage or electrical hazards.
    • Disposal: Follow manufacturer's guidelines for maintenance.
20. Stir Bar Retriever:
    • Handling: Use for retrieving stir bars from containers. Handle carefully to avoid damaging the stir bars.
    • Disposal: If stir bars are damaged, consult with a supervisor.
21. pH Meter:
    • Handling: Calibrate and store according to manufacturer's instructions to ensure accurate measurements. Avoid contact with the electrode unless necessary.
    • Disposal: If damaged, consult with a supervisor. Never dispose of pH electrodes in general waste.
22. 125mL Erlenmeyer Flask:
    • Handling: Use for mixing or heating liquids. Avoid exposure to extreme heat or cold.
    • Disposal: Clean thoroughly after use.
23. Tryptone, Yeast Extract, NaCl, Luria Broth Agar Base:
    • Handling: These reagents are generally non-hazardous but should be handled carefully to avoid contamination.
    • Disposal: Dispose of excess chemicals in designated chemical waste containers.
24. Aluminum Foil:
    • Handling: Handle with clean hands or gloves to avoid contamination.
    • Disposal: Dispose of clean foil in regular waste. If contaminated, dispose of it in a designated hazardous waste container.
25. Petri Dish:
    • Handling: Use for agar plating. Sterilize before and after use.
    • Disposal: Dispose of used or broken petri dishes in designated biohazard waste containers if contaminated.
26. Calcium Chloride (CaCl2), Tris, Boric Acid, EDTA:
    • Handling: Handle with care and wear appropriate PPE. Store in tightly sealed containers.
    • Disposal: Dispose of excess chemicals according to institution-specific waste disposal protocols.
27. Agarose:
    • Handling: Handle with care and avoid inhalation of the powder.
    • Disposal: Dispose of agarose in general waste unless contaminated with hazardous chemicals.
28. MiniONE Agarose Gel Electrophoresis Kit:
    • Handling: Follow the manufacturer’s instructions for setting up and running electrophoresis. Always handle with gloves to avoid contamination.
    • Disposal: Dispose of used gels in biohazard waste containers. Ensure all electrophoresis chemicals are disposed of in chemical waste containers.
29. 6M Hydrochloric Acid (HCl):
    • Handling: Wear gloves, goggles, and lab coat. Always add acid to water (not the other way around) to prevent exothermic reactions.
    • Disposal: Neutralize with a base like sodium bicarbonate before disposal down the drain (ensure this is approved by local regulations).
30. 6M Sodium Hydroxide (NaOH):
    • Handling: Handle with care, as NaOH is a strong base and can cause burns. Always use in a well-ventilated area.
    • Disposal: Neutralize with an acid like hydrochloric acid before disposal in compliance with institutional guidelines.
31. pH Paper:
    • Handling: Store in a dry place. Handle with clean hands to avoid contamination.
    • Disposal: Dispose of used pH paper in general waste.
32. pH Calibration Buffers:
    • Handling: Use freshly prepared or new calibration buffers for accuracy.
    • Disposal: Dispose of used calibration buffers in appropriate chemical waste containers.
33. pH Electrode Storage Solution and Cleaning Solution:
    • Handling: Use only as directed to prevent damage to the electrode.
    • Disposal: Dispose of any excess solution according to institutional hazardous waste procedures.