2.3: Appendix C
- Page ID
- 96199
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)Differentiation of Bacterial Cultures based on Morphological and Physiological Characteristics
Group 1: Aerobic and Anaerobic Cocci
| Gram stain | Arrangement | |
|---|---|---|
| Micrococcus spp. | + | tetrads |
| Neisseria gonorrhoeae | – | diplococci |
| Catalase | Oxidase | Arrangement | Oxygen | |
|---|---|---|---|---|
| Staphylococcus spp (see Table 1) | + | – | clusters | Facultative anaerobe |
| Streptococcus spp.
(see Table 4) |
– | – | chains | Aerotolerant anaerobe |
| Enterococcus spp.
(see Table 4) |
– | – | chains | Aerotolerant anaerobe |
| Staphylococcus aureus | Staphylococcus epidermidis | Staphylococcus saprophyticus | |
|---|---|---|---|
| Coagulase | 4+ | – | 1–2+ |
| Mannitol Salt Agar | + | – | + |
| Urease | – or wk+ | + | + |
| Hemolysis | Beta (β) | – or wk + | – |
| Novobiocin resistance | Susc. (-) | Susc. (-) | Res. (+) |
| Streptococcus pyogenes | Enterococcus faecalis | |
|---|---|---|
| Hemolysis on BAP | Beta (β) | Gamma (γ) |
| Bile Esculin | No growth (-) | Growth/black (+) |
Group 2: Aerobic and Facultatively Anaerobic Bacilli
Gram positive bacilli
Endospore forming: Bacillus spp. (see Table 5)
Non-endospore forming: Corynebacterium xerosis
| Endospore shape, location, sporangium | |
|---|---|
| B. cereus | Oval, central, sporangium not swollen |
| B. lentus | Oval, terminal, sporangium swollen |
Gram negative bacilli
Strictly aerobic: Pseudomonas aeruginosa
Facultatively anaerobic: Enterobacteriaceae (see Table 6)
| TSI | Sulfide/Indole/Motility | Methyl Red | VP | Citrate | |
|---|---|---|---|---|---|
| Escherichia coli | A/A | – /+/+ | + | – | – |
| Enterobacter aerogenes | A/A | -/-/+ | – | + | + |
| Citrobacter freundii | A/A | -/-/+ | + | – | + |
| Klebsiella pneumoniae | A/A | -/-/- | – | + | + |
| Salmonella enteritidis | K/A | +/-/+ | + | – | + |