14.1: Introduction
- Page ID
- 40995
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The purpose of mRNA sequencing (RNA-seq) is to measure the levels of mRNA transcripts for every gene in a given cell. mRNA sequencing was a daunting task, and requires approximately 40 million aligned reads in order to accurately measure mRNA transcripts.This did not become possible until 2009, when next-generation sequencing technologies became more advanced and efficient.
In this chapter, we will explore the different techniques for using mRNA sequencing data to aid in gene and transcript discovery as well as in expression analysis.