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8: Agarose gel electrophoresis

Last updated

Mar 6, 2021
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- 7.8: References
- 8.1: Background

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Agarose gels are used to analyze DNA molecules. These gels are simple to construct, because they rely only on
the gelling properties of agarose. Molecules are separated by size and visualized with fluorescent intercalating
dyes. In this lab, you will analyze the products of the PCR reactions from the previous lab.

Objectives

At the end of this lab, students should be able to:

Prepare an agarose gel for separating DNA molecules.
Separate DNA molecules by electrophoresis.
Visualize DNA molecules on agarose gels using intercalating dyes.
Estimate the approximate sizes of DNA molecules using size standards.

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