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- https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/16%3A_Write_It_Up/16.02%3A_Micro-report_1-_Selective_plating_experimentMaterials and Methods: In preparing the M&M, ask yourself “What information will an investi- gator need to reproduce our experiments?” Provide information on the strains and media that you used, as we...Materials and Methods: In preparing the M&M, ask yourself “What information will an investi- gator need to reproduce our experiments?” Provide information on the strains and media that you used, as well as the procedures that you used for spot plating. Describe the growth of the met deletion strains on the various media and include your preliminary strain identifications from the experimental data.
- https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/10%3A_Plasmids/10.05%3A_ReferencesJohnston M (1987) A model fungal regulatory mechanism: the GAL1 genes of Saccharomyces cerevisiae. Microbiol Rev 51: 458-476.
- https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/03%3A_Meet_the_yeastIn this lab, you will learn to adjust the light microscope using a human blood smear as the specimen. You will then use the microscope to analyze cultures of the budding yeast, Saccharomyces cerevisia...In this lab, you will learn to adjust the light microscope using a human blood smear as the specimen. You will then use the microscope to analyze cultures of the budding yeast, Saccharomyces cerevisiae, and the fission yeast, Schizosaccharomyces pombe. Anton van Leeuwenhoek was the first to observe human blood cells and to observe yeast and bacteria, which he referred
- https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/01%3A_Introduction/1.04%3A_AcknowledgmentsDouglas Warner has helped to design the experiments and has contributed many revisions to the text. Over the past five years, many teaching assistants have provided able leadership for their sections ...Douglas Warner has helped to design the experiments and has contributed many revisions to the text. Over the past five years, many teaching assistants have provided able leadership for their sections and offered valuable feedback about the effectiveness of this manual and suggestions Special thanks are due to David Chou, Class of 2012, who designed the cover and layout of this manual.
- https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/12%3A_Yeast_Transformation/12.06%3A_Exercise_2_-_Replica_plating_and_complementationIt is important to have a light touch during replica plating!! The goal is to transfer a small portion of cells from each colony on the master plate (the plates carrying your transfor- mants) to a num...It is important to have a light touch during replica plating!! The goal is to transfer a small portion of cells from each colony on the master plate (the plates carrying your transfor- mants) to a number of plates containing different media. Remove the lid from your master plate and invert the plate on the block, aligning the orienta- tion marker on the plate with the marker on the block. GENTLY and EVENLY tap on the bot- tom of the plate to transfer cells to the velveteen.
- https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/06%3A_Analysis_of_mutant_strains/6.06%3A_Exercise_1_-_Predicting_growth_properties_of_mutant_strainsYC Complete medium also contains methionine and sulfate, so we will use “dropout” media to test the ability of strains to grow on various sulfur sources. Predict the ability of met mutants to grow on ...YC Complete medium also contains methionine and sulfate, so we will use “dropout” media to test the ability of strains to grow on various sulfur sources. Predict the ability of met mutants to grow on various sulfur sources and complete the table below. Place a plus (+) when you predict that the strain will grow on the plate and a minus (-) when you do not expect the strain to grow.
- https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/13%3A_Protein_overexpression/13.05%3A_ReferencesCold Spring Harbor Laboratory Press, Cold Spring Harbor. Buser C & Drubin DG (2013) Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immun...Cold Spring Harbor Laboratory Press, Cold Spring Harbor. Buser C & Drubin DG (2013) Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling. Gelperin DM, White MA, Wilkinson ML et al. (2005) Biochemical and genetic analysis of the yeast proteome with a movable ORF collection. Johnston M (1987) A model fungal regulatory mechanism: the GAL1 genes of Saccharomyces cerevisiae. Sherman F (2002) Getting started with yeast.
- https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/05%3A_Introduction_to_databases/5.02%3A_From_the_research_bench_to_the_databaseIn preparation for the manuscript submission (reviewers of the manuscript will want to see the accession numbers), the researcher plans to submit data to three different databases: a nucleotide databa...In preparation for the manuscript submission (reviewers of the manuscript will want to see the accession numbers), the researcher plans to submit data to three different databases: a nucleotide database, a structure database and an organism database. The number of entries in the PDB databases is orders of magnitude smaller than the number of predicted proteins in GenBank, reflecting the difficulties inherent in determining structures of macromolecules.
- https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/08%3A_Agarose_gel_electrophoresisAgarose gels are used to analyze DNA molecules. the gelling properties of agarose. Molecules are separated by size and visualized with fluorescent intercalating In this lab, you will analyze the produ...Agarose gels are used to analyze DNA molecules. the gelling properties of agarose. Molecules are separated by size and visualized with fluorescent intercalating In this lab, you will analyze the products of the PCR reactions from the previous lab. At the end of this lab, students should be able to: Prepare an agarose gel for separating DNA molecules. Visualize DNA molecules on agarose gels using intercalating dyes. Estimate the approximate sizes of DNA molecules using size standards.
- https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/13%3A_Protein_overexpression/13.06%3A_Section_6-
- https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/08%3A_Agarose_gel_electrophoresis/8.05%3A_Stain_and_analyze_the_agarose_gelRemove the gel from the apparatus and transfer the gel to a small tray. With a spatula, carefully place the gel on the transilluminator and close the cover to the Gel- Logic apparatus. (Drain the wash...Remove the gel from the apparatus and transfer the gel to a small tray. With a spatula, carefully place the gel on the transilluminator and close the cover to the Gel- Logic apparatus. (Drain the wash solution into the waste container.) Turn on the transilluminator light and photograph the gel according to the posted instructions. Determine the approximate length of the DNA molecules in your samples by comparing their migration to that of the standards.
