The growth properties of mutant strains can often provide information about the gene products involved in biochemical pathways within cells. The sulfur containing amino acids, Met and Cys, are synthesized in a multi-step pathway in yeast. In this lab, you will use selective and differential media to determine which MET genes have been inactivated in S. cerevisiae met deletion strains.
At the end of this lab, students should be able to:
- use correct genetic nomenclature for genes, proteins and mutants in written reports.
- explain how genetic screens are used to isolate mutants with particular phenotypes.
- distinguish met strains by their ability to grow on selective media containing various sulfur sources.
- predict how mutations in the genes involved in Met and Cys synthesis will affect the concentrations of metabolites in the pathway.