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OER @ City Tech Biology

Open educational resources (OER) are free and (re)distributable teaching materials. This site is a sponsored by the Ursula Schwerin Library to select and curate resources for use in General Biology 1. This was extended to include Molecular & Cell Biology through the U.S. Department of Education Title V Grant, “A Living Lab“. This site was originally authored and curated by Jeremy Seto, Department of Biological Sciences – New York City College of Technology. In the spirit of remixing and redistribution, the entire WordPress site can be downloaded from GitHub as well as customized imagery (https://github.com/jeremyseto/bio-oer).

The NYCCT General Biology 1 Lab will follow the sequence below:

WEEK	EXERCISE
I	Microscope: Basics Scientific Method Reporting in Science
II	Chromatography Measurements in Science: Metric System Quantitative Skills
III	Chemistry of Water Atoms Water pH: Acids, Bases, and Buffers (Activity)
IV	Biologically Important Molecules I: Carbohydrates & Lipids Basic Organic Chemistry Carbohydrates Activity
V	Biologically Important Molecules II: Proteins & Nucleic Acids Protein Activity Purification of DNA, Dische’s Test Review (Concept Map)
VI	Quantitative Determination of Proteins Beer’s Law (virtual) Protein Assay (SpectroVis)
VII	Membranes and Biological Transport: Diffusion and Osmosis Size Gummy Cell Models Dialysis
VIII	Enzyme and Energy Enzyme Kinetics activity (SpectroVis Plus)
IX	Cellular Energy Anaerobic and Aerobic Review (Concept)
X	Photosynthesis Pigments Absorbance Spectra and the role of Light in Carbohydrate Synthesis Review (Concept)
XI	Cell Division Mitosis: Estimating time of phases Chromosome Modeling Meiosis: Reductional Division and Gametogenesis Comparing Cell Division Chromosomes and Karyotypes
XII	Genetics Genetics of Taste Activity Sex-Linked Genes Co-Dominance and Multiple Alleles Probability and Statistics with Chi-Square
XIII	Principles of Gel Electrophoresis Analyzing DNA Restriction Enzymes
XIV	DNA Fingerprinting RFLP PCR Technology Forensics
XV	Hardy-Weinberg and Population Genetics Simulations Quiz

Molecular and Cell Biology Lab Schedule:

For additional background material, consult the “Reading Pages” that correspond to the textbook: Essential Cell Biology 4th By Alberts et al.

Week	Lab Schedule	Reading Pages
1	Micropipetting and centrifuges Metric Conversions and Scientific measures
2	Plasmid Purification by Alkaline lysis Agarose Gel Electrophoresis Plasmid Identification using restriction enzymes In silico Digestion	Alberts 327-338, 347
3	Bacterial transformation: heat shock History of DNA as genetic material pGlo/Genotype to Phenotype → Hypothesis test/Predictions	Alberts 174-176
4	Quiz 1 Plasmid Structure → Visualize transformation predictions Transcriptional Control	Alberts 248-249 Alberts 331-333
5	DNA isolation from Eukaryotes Introduction to DNA replication and PCR Genetics Review	Alberts 200-202
6	Crime scene PCR VNTR, STRs, RFLP PTC Paper and human alleles (SNP)	Alberts 340-344 Alberts 664 Alberts 674-677
7	Tracing Origins Mitochondrial Haplotyping (activity) Using Transposons for lineage with Alu insertion	Alberts 302-303,314 Alberts 486-487
8	Quiz 2 Barcoding of Life (COI and RbcL activity) Tissue Culture demonstration
9	Introduction to Tissue Culture Gene Expression Prokaryotic transcription Eukaryotic mRNA RNA isolation and Reverse Transcription
10	Quantitative Real-Time PCR Sequencing technology (Sanger)	Alberts 343-347
11	Quiz 3 Bioinformatics: Analysis of mitochondrial DNA and Barcoding Primer Design for qPCR
12	Sequencing technology (Next Generation Sequencing) Illumina Ion Torrent PacBio MinION qPCR results review	Alberts 335-339
13	Gene Cloning Workflow Transgenic Technology and activity Transfection of Eukaryotic cells (demonstration)	Alberts 351-353
14	GFP Protein purification Introducing biotechnology: Protein Production Protein Purification (activity) Review Transfection
15	Quiz 4

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OER @ City Tech Biology

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Molecular and Cell Biology Lab Schedule: