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Biology LibreTexts

Biology Open Educational Resources

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OER @ City Tech Biology

Open educational resources (OER) are free and (re)distributable teaching materials. This site is a sponsored by the Ursula Schwerin Library to select and curate resources for use in General Biology 1. This was extended to include Molecular & Cell Biology through the U.S. Department of Education Title V Grant, “A Living Lab“. This site was originally authored and curated by Jeremy Seto, Department of Biological Sciences – New York City College of Technology. In the spirit of remixing and redistribution, the entire WordPress site can be downloaded from GitHub as well as customized imagery (https://github.com/jeremyseto/bio-oer).

Table of Contents

The NYCCT General Biology 1 Lab will follow the sequence below:

WEEK EXERCISE
I
  • Microscope: Basics
  • Scientific Method
  • Reporting in Science
II Chromatography
  • Measurements in Science: Metric System
  • Quantitative Skills
III Chemistry of Water
  • Atoms
  • Water
  • pH: Acids, Bases, and Buffers (Activity)
IV Biologically Important Molecules I: Carbohydrates & Lipids
  • Basic Organic Chemistry
  • Carbohydrates Activity
V Biologically Important Molecules II: Proteins & Nucleic Acids
  • Protein Activity
  • Purification of DNA, Dische’s Test
  • Review (Concept Map)
VI Quantitative Determination of Proteins
  • Beer’s Law (virtual)
  • Protein Assay (SpectroVis)
VII Membranes and Biological Transport: Diffusion and Osmosis
  • Size
  • Gummy
  • Cell Models
  • Dialysis
VIII Enzyme and Energy
  • Enzyme Kinetics activity (SpectroVis Plus)
IX Cellular Energy
  • Anaerobic and Aerobic
  • Review (Concept)
X Photosynthesis
  • Pigments
  • Absorbance Spectra and the role of Light in Carbohydrate Synthesis
  • Review (Concept)
XI Cell Division
  • Mitosis: Estimating time of phases
    • Chromosome Modeling
  • Meiosis: Reductional Division and Gametogenesis
  • Comparing Cell Division
  • Chromosomes and Karyotypes
XII Genetics
  • Genetics of Taste Activity
  • Sex-Linked Genes
  • Co-Dominance and Multiple Alleles
  • Probability and Statistics with Chi-Square
XIII Principles of Gel Electrophoresis
  • Analyzing DNA
  • Restriction Enzymes
XIV DNA Fingerprinting
  • RFLP
  • PCR Technology
  • Forensics
XV Hardy-Weinberg and Population Genetics Simulations

Quiz

Molecular and Cell Biology Lab Schedule:

For additional background material, consult the “Reading Pages” that correspond to the textbook: Essential Cell Biology 4th By Alberts et al.

Week Lab Schedule Reading Pages
1
  • Micropipetting and centrifuges
  • Metric Conversions and Scientific measures
2
  • Plasmid Purification by Alkaline lysis
  • Agarose Gel Electrophoresis
  • Plasmid Identification using restriction enzymes
  • In silico Digestion
Alberts 327-338, 347
3
  • Bacterial transformation: heat shock
  • History of DNA as genetic material
  • pGlo/Genotype to Phenotype → Hypothesis test/Predictions
Alberts 174-176
4 Quiz 1
  • Plasmid Structure → Visualize transformation predictions
  • Transcriptional Control
Alberts 248-249

Alberts 331-333

5
  • DNA isolation from Eukaryotes
  • Introduction to DNA replication and PCR
  • Genetics Review
Alberts 200-202
6 Crime scene PCR
  • VNTR, STRs, RFLP
  • PTC Paper and human alleles (SNP)
Alberts 340-344
Alberts 664
Alberts 674-677
7 Tracing Origins
  • Mitochondrial Haplotyping (activity)
  • Using Transposons for lineage with Alu insertion
Alberts 302-303,314
Alberts 486-487
8
  • Quiz 2
  • Barcoding of Life (COI and RbcL activity)
  • Tissue Culture demonstration
9 Introduction to Tissue Culture
  • Gene Expression
  • Prokaryotic transcription
  • Eukaryotic mRNA
  • RNA isolation and Reverse Transcription
10
  • Quantitative Real-Time PCR
  • Sequencing technology (Sanger)
Alberts 343-347
11
  • Quiz 3
  • Bioinformatics: Analysis of mitochondrial DNA and Barcoding
  • Primer Design for qPCR
12 Sequencing technology (Next Generation Sequencing)
  • Illumina
  • Ion Torrent
  • PacBio
  • MinION
  • qPCR results review
Alberts 335-339
13 Gene Cloning Workflow
  • Transgenic Technology and activity
  • Transfection of Eukaryotic cells (demonstration)
Alberts 351-353
14 GFP Protein purification
  • Introducing biotechnology: Protein Production
  • Protein Purification (activity)
  • Review Transfection
15 Quiz 4
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