- differentiate among competitive, uncompetitive, and mixed inhibition of enzymes by reversible, noncovalent inhibitors by writing coupled chemical equilibria equations and drawing cartoons showing molecular interactions among, E, S, and I;
- using LeChatelier's principle and coupled chemical equilibria equations, draw double reciprocal (Lineweaver-Burk plots) and semilog plots for enzyme catalyzed reactions in the presence of different fixed concentrations of inhibitors and activators of enzyme
- define KIS and KII for competitive, uncompetitive, and mixed inhibition from coupled chemical equilibria and double reciprocal plots;
- differentiate between apparent and actual dissociations constants constants of an inhibitor and enzyme from double reciprocal plots and equations initial rate mathematical equations;
- define agonist, partial agonist, antagonist, and mixed (noncompetitive antagonists) from analogy to enzymes and their inhibitors;
- describe different ways that pH changes could affect the activity of an enzyme and suggest how each could affect Km and kcat.