Skills to Develop
- state the charge on amino acid side chains using the Henderson Hasselbach equation and the approximate charge by inspection at any given pH
- draw mechanisms and identify products for the reaction of nucleophilic side chains Lys and Cys with common chemical modification agents and extend this understanding to reactions of His.
- draw mechanisms for disulfide exchange reactions for sulfhydryls using them and oxidation numbers to explain redox reactions of cysteine/cystine.
Thumbnail: Structure of human hemoglobin. The proteins α and β subunits are in red and blue, and the iron-containing heme groups in green. From PDB: 1GZX. Image used with permission (GNU; Proteopedia Hemoglobin).