This page discusses the three-point cross method for mapping three linked genes simultaneously. It involves crossing pure breeding lines to create a trihybrid and testcrossing to observe phenotypic di...This page discusses the three-point cross method for mapping three linked genes simultaneously. It involves crossing pure breeding lines to create a trihybrid and testcrossing to observe phenotypic distributions, allowing for the identification of rare double crossover events. The method aids in calculating recombination frequencies, correcting for undetected double crossovers, and improving genetic mapping accuracy.
This page explains how trihybrid test crosses in corn are more effective than dihybrid tests for determining gene order and physical distances between linked alleles. It discusses the importance of re...This page explains how trihybrid test crosses in corn are more effective than dihybrid tests for determining gene order and physical distances between linked alleles. It discusses the importance of recombination percentages and the impact of double recombinants on mapping accuracy. The text emphasizes that closer allelic intervals lead to more precise genetic maps and outlines the methodology for discerning gene order via rare crossover classes.
