This page discusses the three-point cross method for mapping three linked genes simultaneously. It involves crossing pure breeding lines to create a trihybrid and testcrossing to observe phenotypic di...This page discusses the three-point cross method for mapping three linked genes simultaneously. It involves crossing pure breeding lines to create a trihybrid and testcrossing to observe phenotypic distributions, allowing for the identification of rare double crossover events. The method aids in calculating recombination frequencies, correcting for undetected double crossovers, and improving genetic mapping accuracy.
A particularly efficient method of mapping three genes at once is the three-point cross, which allows the order and distance between three potentially linked genes to be determined in a single cross e...A particularly efficient method of mapping three genes at once is the three-point cross, which allows the order and distance between three potentially linked genes to be determined in a single cross experiment. This is particularly useful when mapping a new mutation with an unknown location to two previously mapped loci.
