This page explores the difficulties of inferring allele arrangements in genetic studies without direct chromosome analysis. It highlights the importance of parental genotypes in determining allele con...This page explores the difficulties of inferring allele arrangements in genetic studies without direct chromosome analysis. It highlights the importance of parental genotypes in determining allele configurations (cis or trans) and details an experiment with dihybrid organisms from pure-breeding lines. A testcross with recessive individuals helps identify gamete genotypes, enabling calculation of recombination frequencies from observed phenotypes.