PCR is carried out in a test tube with the DNA template, primers specific for the region that is desired, DNA polymerase, and reagents that stabilize the reaction. As you dispense the Master mix, inse...PCR is carried out in a test tube with the DNA template, primers specific for the region that is desired, DNA polymerase, and reagents that stabilize the reaction. As you dispense the Master mix, insert the micropipette tip into the mix so that you actually see the small volume go directly into the mix. Open the package containing the gel and insert the gel (with the come in place) into the apparatus right edge first.