Grasp the cap with the pinky and ring finger of your dominate hand and gently twist the tube out of the cap keeping your dominate hand still. Begin with the loop at the bottom of the slant you are tra...Grasp the cap with the pinky and ring finger of your dominate hand and gently twist the tube out of the cap keeping your dominate hand still. Begin with the loop at the bottom of the slant you are transferring bacteria to and snake the loop up the surface of the slant. Grasp the cap with the pinky and ring finger of your dominate hand and gently twist the tube out of the cap.