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- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/11%3A_GenomicsThis page covers essential topics in genetic engineering and molecular biology, such as recombinant DNA, PCR, gene therapy, and transgenic organisms. It discusses techniques like RFLPs, gel blotting, ...This page covers essential topics in genetic engineering and molecular biology, such as recombinant DNA, PCR, gene therapy, and transgenic organisms. It discusses techniques like RFLPs, gel blotting, and antisense RNA, emphasizing their importance in understanding genetic disorders and genetic screening. Additionally, it contrasts forward and reverse genetics while introducing metagenomics to highlight the study of unknown microorganisms.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/11%3A_Genomics/11.01%3A_Recombinant_DNA_and_Gene_CloningThis page explains the creation and application of recombinant DNA (rDNA) by combining DNA from different sources using enzymes, enabling cloning in organisms like E. coli. It outlines the use of plas...This page explains the creation and application of recombinant DNA (rDNA) by combining DNA from different sources using enzymes, enabling cloning in organisms like E. coli. It outlines the use of plasmids with antibiotic resistance genes for selection.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/11%3A_Genomics/11.05%3A_Transgenic_AnimalsThis page discusses the creation and application of transgenic animals using recombinant DNA technology for research and therapy. It highlights various methods, including the development of knockout a...This page discusses the creation and application of transgenic animals using recombinant DNA technology for research and therapy. It highlights various methods, including the development of knockout and "knock-in" mice, alongside successful transgenic sheep, goats, chickens, pigs, and primates. These animals can produce therapeutic proteins, exemplified by sheep producing human proteins in milk and chickens capable of cost-effective protein production in eggs.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/11%3A_Genomics/11.06%3A_Transgenic_PlantsThis page discusses advancements in genetic engineering of plants through recombinant DNA technology, allowing for rapid trait introduction compared to traditional methods. Techniques like plasmids an...This page discusses advancements in genetic engineering of plants through recombinant DNA technology, allowing for rapid trait introduction compared to traditional methods. Techniques like plasmids and gene guns have led to achievements such as enhanced nutritional quality, pest resistance, and herbicide tolerance. Despite controversies surrounding ecological risks and "terminator" genes, transgenic crops are widely adopted in the U.S. for their benefits, particularly in herbicide resistance.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/16%3A_The_Anatomy_and_Physiology_of_Plants/16.03%3A_Reproduction_in_Plants/16.3F%3A_Transgenic_PlantsThis page discusses advancements in recombinant DNA technology for plant genetic modification, highlighting methods like plasmid vectors and gene bombardment. Notable successes include nutritional enh...This page discusses advancements in recombinant DNA technology for plant genetic modification, highlighting methods like plasmid vectors and gene bombardment. Notable successes include nutritional enhancements, resistance to pests and herbicides, and developments like "terminator" genes. Transgenic plants are also being investigated for biopharmaceutical production. While there are ecological concerns, the adoption of genetically modified crops remains substantial, particularly in the U.S.
