The enzyme primase catalyzes the synthesis of the primers from which DNA polymerases can begin synthesis (Figure 5.21). Primers are short oligonucleotides, ranging from 6 to 60 nucleotides long. They ...The enzyme primase catalyzes the synthesis of the primers from which DNA polymerases can begin synthesis (Figure 5.21). Primers are short oligonucleotides, ranging from 6 to 60 nucleotides long. They can be made of ribonucleotides or a mixture of deoxyribonucleotides and ribonucleotides. The principal primase in E. coli is the 60 kDa protein called DnaG protein, the product of the dnaG gene.
The page provides an in-depth exploration of DNA replication, including its mechanisms, regulation, and interaction with chromatin. The semiconservative model of DNA replication, demonstrated by the M...The page provides an in-depth exploration of DNA replication, including its mechanisms, regulation, and interaction with chromatin. The semiconservative model of DNA replication, demonstrated by the Meselson-Stahl experiment, is explained, emphasizing the roles of essential enzymes like DNA polymerases, ligase, and helicase. Differences between prokaryotic and eukaryotic DNA replication are discussed, focusing on the regulatory processes involved in each system.
