This page discusses DNA sequencing and its techniques, including dideoxy sequencing, which uses modified nucleotides to determine sequences, and capillary electrophoresis for fragment analysis. It hig...This page discusses DNA sequencing and its techniques, including dideoxy sequencing, which uses modified nucleotides to determine sequences, and capillary electrophoresis for fragment analysis. It highlights next-generation sequencing methods like Illumina, which improve speed and cost-effectiveness by sequencing multiple short templates at once but notes the limitation of these short lengths in certain applications.
This page describes the polymerase chain reaction (PCR), a rapid DNA cloning method that involves synthesizing primers, heat-induced strand separation, and polymerization with heat-stable DNA polymera...This page describes the polymerase chain reaction (PCR), a rapid DNA cloning method that involves synthesizing primers, heat-induced strand separation, and polymerization with heat-stable DNA polymerase. Each cycle doubles the DNA amount, enabling exponential amplification, which can produce billions of copies quickly through automation. PCR is effective for analyzing limited DNA samples but is sensitive to contamination.
