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- https://bio.libretexts.org/Courses/Clackamas_Community_College/Clackamas_Biology_112_OER_Textbook_(OpenStax)/08%3A_Molecular_Biology/8.02%3A_DNA_ReplicationWhen a cell divides, it is important that each daughter cell receives an identical copy of the DNA. This is accomplished by the process of DNA replication. The replication of DNA occurs during the syn...When a cell divides, it is important that each daughter cell receives an identical copy of the DNA. This is accomplished by the process of DNA replication. The replication of DNA occurs during the synthesis phase, or S phase, of the cell cycle, before the cell enters mitosis or meiosis.
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Microbiology_OER_-_Ying_Liu/13%3A_DNA_Replication_Transcription_and_Translation/13.03%3A_RNA_TranscriptionDuring the process of transcription, the information encoded within the DNA sequence of one or more genes is transcribed into a strand of RNA, also called an RNA transcript. The resulting single-stran...During the process of transcription, the information encoded within the DNA sequence of one or more genes is transcribed into a strand of RNA, also called an RNA transcript. The resulting single-stranded RNA molecule, composed of ribonucleotides containing the bases adenine, cytosine, guanine, and uracil, acts as a mobile molecular copy of the original DNA sequence. Transcription in prokaryotes and in eukaryotes requires the DNA double helix to partially unwind in the region of RNA synthesis.
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Genetics/07%3A_DNA_Structure_and_Function/7.05%3A_DNA_Replication_in_ProkaryotesDNA replication has been extremely well studied in prokaryotes primarily because of the small size of the genome and the mutants that are available. E. coli has 4.6 million base pairs in a single circ...DNA replication has been extremely well studied in prokaryotes primarily because of the small size of the genome and the mutants that are available. E. coli has 4.6 million base pairs in a single circular chromosome and all of it gets replicated in approximately 42 minutes, starting from a single origin of replication and proceeding around the circle in both directions. This means that approximately 1000 nucleotides are added per second. The process is rapid and occurs without many mistakes.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Map%3A_Raven_Biology_12th_Edition/14%3A_DNA-_The_Genetic_Material/14.04%3A_Prokaryotic_Replication/14.4.01%3A_DNA_Replication_in_ProkaryotesDNA replication has been extremely well studied in prokaryotes primarily because of the small size of the genome and the mutants that are available. E. coli has 4.6 million base pairs in a single circ...DNA replication has been extremely well studied in prokaryotes primarily because of the small size of the genome and the mutants that are available. E. coli has 4.6 million base pairs in a single circular chromosome and all of it gets replicated in approximately 42 minutes, starting from a single origin of replication and proceeding around the circle in both directions. This means that approximately 1000 nucleotides are added per second. The process is quite rapid and occurs without many mistake
- https://bio.libretexts.org/Courses/Mansfield_University_of_Pennsylvania/BSC_3271%3A_Microbiology_for_Health_Sciences_Sp21_(Kagle)/08%3A_DNA_Replication/8.03%3A_DNA_ReplicationThe DNA replication process is semiconservative, which results in two DNA molecules, each having one parental strand of DNA and one newly synthesized strand. In bacteria, the initiation of replication...The DNA replication process is semiconservative, which results in two DNA molecules, each having one parental strand of DNA and one newly synthesized strand. In bacteria, the initiation of replication occurs at the origin of replication, where supercoiled DNA is unwound by DNA gyrase, made single-stranded by helicase, and bound by single-stranded binding protein to maintain its single-stranded state.
- https://bio.libretexts.org/Bookshelves/Genetics/Online_Open_Genetics_(Nickle_and_Barrette-Ng)/08%3A_Techniques_of_Molecular_Genetics/8.03%3A__Isolating_or_Detecting_a_Specific_Sequence_by_PCRThe Polymerase Chain Reaction (PCR) is a method of DNA replication that is performed in a test tube (i.e. in vitro). Here “polymerase” refers to a DNA polymerase enzyme extracted and purified from bac...The Polymerase Chain Reaction (PCR) is a method of DNA replication that is performed in a test tube (i.e. in vitro). Here “polymerase” refers to a DNA polymerase enzyme extracted and purified from bacteria, and “chain reaction” refers to the ability of this technique produce millions of copies of a DNA molecule, by using each newly replicated double helix as a template to synthesize two new DNA double helices. PCR is therefore a very efficient method of amplifying DNA.
- https://bio.libretexts.org/Workbench/Bio_11A_-_Introduction_to_Biology_I/17%3A_DNA_Replication/17.03%3A_DNA_Replication_in_ProkaryotesThe nicks that remain between the newly synthesized DNA (that replaced the RNA primer) and the previously synthesized DNA are sealed by the enzyme DNA ligase, which catalyzes the formation of phosphod...The nicks that remain between the newly synthesized DNA (that replaced the RNA primer) and the previously synthesized DNA are sealed by the enzyme DNA ligase, which catalyzes the formation of phosphodiester linkages between the 3'-OH end of one nucleotide and the 5' phosphate end of the other fragment.
- https://bio.libretexts.org/Courses/Cosumnes_River_College/Contemporary_Biology_(Aptekar)/07%3A_Molecular_Biology_and_Biotechnology/7.02%3A_DNA_ReplicationWhen a cell divides, it is important that each daughter cell receives an identical copy of the DNA. This is accomplished by the process of DNA replication. The replication of DNA occurs during the syn...When a cell divides, it is important that each daughter cell receives an identical copy of the DNA. This is accomplished by the process of DNA replication. The replication of DNA occurs during the synthesis phase, or S phase, of the cell cycle, before the cell enters mitosis or meiosis.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/General_Biology_2e_(OpenStax)/03%3A_Unit_III-_Genetics/3.04%3A_DNA_Structure_and_Function/3.4.05%3A_DNA_Replication_in_ProkaryotesDNA replication has been extremely well studied in prokaryotes primarily because of the small size of the genome and the mutants that are available. E. coli has 4.6 million base pairs in a single circ...DNA replication has been extremely well studied in prokaryotes primarily because of the small size of the genome and the mutants that are available. E. coli has 4.6 million base pairs in a single circular chromosome and all of it gets replicated in approximately 42 minutes, starting from a single origin of replication and proceeding around the circle in both directions. This means that approximately 1000 nucleotides are added per second. The process is rapid and occurs without many mistakes.
- https://bio.libretexts.org/Courses/Norco_College/OpenStax_Biology_2e_for_Norco_College/14%3A_DNA_Structure_and_Function/14.05%3A_DNA_Replication_in_ProkaryotesDNA replication has been extremely well studied in prokaryotes primarily because of the small size of the genome and the mutants that are available. E. coli has 4.6 million base pairs in a single circ...DNA replication has been extremely well studied in prokaryotes primarily because of the small size of the genome and the mutants that are available. E. coli has 4.6 million base pairs in a single circular chromosome and all of it gets replicated in approximately 42 minutes, starting from a single origin of replication and proceeding around the circle in both directions. This means that approximately 1000 nucleotides are added per second. The process is rapid and occurs without many mistakes.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Map%3A_Raven_Biology_12th_Edition/17%3A_Biotechnology/17.02%3A_Amplifying_DNA_Using_the_Polymerase_Chain_ReactionThe polymerase chain reaction is a technique for quickly "cloning" a particular piece of DNA in the test tube (rather than in living cells like E. coli). Thanks to this procedure, one can make virtual...The polymerase chain reaction is a technique for quickly "cloning" a particular piece of DNA in the test tube (rather than in living cells like E. coli). Thanks to this procedure, one can make virtually unlimited copies of a single DNA molecule even though it is initially present in a mixture containing many different DNA molecules.
