The abundance of mRNA of a particular gene is usually correlated with the activity of that gene. Fluorescently labeled molecules derived from the transcripts of two tissue samples are hybridized to im...The abundance of mRNA of a particular gene is usually correlated with the activity of that gene. Fluorescently labeled molecules derived from the transcripts of two tissue samples are hybridized to immobilized DNA molecules on the surface of an array. Thus, spots on the micoarray that are more green than red represent genes that are more abundant in the tissue sample from which green-labeled molecules were derived. (Original-Deyholos-CC:AN)
This page discusses functional genomics and its techniques for determining gene functions post-genome identification, highlighting microarray analysis as a key method. Microarrays measure mRNA abundan...This page discusses functional genomics and its techniques for determining gene functions post-genome identification, highlighting microarray analysis as a key method. Microarrays measure mRNA abundance across numerous genes, indicating gene activity and expression variations in different tissues and conditions, including development stages and responses to external factors. They visualize transcript levels with fluorescence, revealing patterns of tissue-specific or temporal gene expression.