If provided in Appendix B3, confirm that the numbering of the nucleotide substitution falls within the codon determined above (here 1189-1191), and use a translator tool to ensure that the bp change r...If provided in Appendix B3, confirm that the numbering of the nucleotide substitution falls within the codon determined above (here 1189-1191), and use a translator tool to ensure that the bp change results in the expected amino acid substitution. If the Tm is less than 78 ºC, increase the length of your primer or increase the % G/C content to increase the T m . However, make sure your primer does not exceed 45 bases.
