Search
- https://bio.libretexts.org/Courses/New_England_College/Microbiology_with_NEC/09%3A_Modern_Applications_of_Microbial_Genetics/9.02%3A_Visualizing_and_Characterizing_DNAFinding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complemen...Finding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complementary single-stranded nucleic acid in the sample. Agarose gel electrophoresis allows for the separation of DNA molecules based on size. Restriction fragment length polymorphism (RFLP) analysis allows for the visualization by agarose gel electrophoresis of distinct variants of a DNA sequence.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/General_Biology_1e_(OpenStax)/3%3A_Genetics/14%3A_DNA_Structure_and_Function/14.2%3A_DNA_Structure_and_SequencingThe building blocks of DNA are nucleotides. The important components of the nucleotide are a nitrogenous base, deoxyribose (5-carbon sugar), and a phosphate group. The nucleotide is named depending on...The building blocks of DNA are nucleotides. The important components of the nucleotide are a nitrogenous base, deoxyribose (5-carbon sugar), and a phosphate group. The nucleotide is named depending on the nitrogenous base. The nitrogenous base can be a purine such as adenine (A) and guanine (G), or a pyrimidine such as cytosine (C) and thymine (T).
- https://bio.libretexts.org/Bookshelves/Biochemistry/Book%3A_Biochemistry_Free_For_All_(Ahern_Rajagopal_and_Tan)/08%3A_Basic_Techniques/8.03%3A_ElectrophoresisElectrophoresis uses an electric field applied across a gel matrix to separate large molecules such as DNA, RNA, and proteins by charge and size. Samples are loaded into the wells of a gel matrix that...Electrophoresis uses an electric field applied across a gel matrix to separate large molecules such as DNA, RNA, and proteins by charge and size. Samples are loaded into the wells of a gel matrix that can separate molecules by size and an electrical field is applied across the gel. This field causes negatively charged molecules to move towards the positive electrode. The gel matrix, itself, acts as a sieve, through which the smallest molecules pass rapidly, while longer molecules are slower-movi
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Genetics/14%3A_Genomics_and_Systems_Biology/14.02%3A_DNA_SequencingThis page discusses DNA sequencing and its techniques, including dideoxy sequencing, which uses modified nucleotides to determine sequences, and capillary electrophoresis for fragment analysis. It hig...This page discusses DNA sequencing and its techniques, including dideoxy sequencing, which uses modified nucleotides to determine sequences, and capillary electrophoresis for fragment analysis. It highlights next-generation sequencing methods like Illumina, which improve speed and cost-effectiveness by sequencing multiple short templates at once but notes the limitation of these short lengths in certain applications.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/09%3A_Regulation_of_Gene_Expression/9.07%3A_FootprintingThis page explains the technique of footprinting, which is used to identify DNA sequences where DNA-binding proteins attach. It involves cloning the DNA with the binding site, labeling it, and digesti...This page explains the technique of footprinting, which is used to identify DNA sequences where DNA-binding proteins attach. It involves cloning the DNA with the binding site, labeling it, and digesting it with DNase I to create radioactive fragments. Protein-bound regions, such as the lac repressor, remain undigested, resulting in gaps on an autoradiogram. By comparing these gaps to a DNA sequencing ladder, the specific base sequence of the operator can be determined.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/11%3A_Genomics/11.08%3A_Gel_BlottingThis page discusses gel blotting, a method to visualize specific macromolecules such as proteins and DNA/RNA through electrophoresis and transfer onto a nitrocellulose filter. The Southern Blot, creat...This page discusses gel blotting, a method to visualize specific macromolecules such as proteins and DNA/RNA through electrophoresis and transfer onto a nitrocellulose filter. The Southern Blot, created by E. M. Southern, identifies DNA fragments using radiolabeled probes. Analogous techniques for RNA and proteins are known as Northern and Western blots, employing similar procedures for detection.
- https://bio.libretexts.org/Courses/Norco_College/OpenStax_Biology_2e_for_Norco_College/14%3A_DNA_Structure_and_Function/14.03%3A_DNA_Structure_and_SequencingThe building blocks of DNA are nucleotides. The important components of the nucleotide are a nitrogenous base, deoxyribose (5-carbon sugar), and a phosphate group. The nucleotide is named depending on...The building blocks of DNA are nucleotides. The important components of the nucleotide are a nitrogenous base, deoxyribose (5-carbon sugar), and a phosphate group. The nucleotide is named depending on the nitrogenous base. The nitrogenous base can be a purine such as adenine (A) and guanine (G), or a pyrimidine such as cytosine (C) and thymine (T).
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Genetics/07%3A_DNA_Structure_and_Function/7.03%3A_DNA_Structure_and_SequencingThe building blocks of DNA are nucleotides. The important components of the nucleotide are a nitrogenous base, deoxyribose (5-carbon sugar), and a phosphate group. The nucleotide is named depending on...The building blocks of DNA are nucleotides. The important components of the nucleotide are a nitrogenous base, deoxyribose (5-carbon sugar), and a phosphate group. The nucleotide is named depending on the nitrogenous base. The nitrogenous base can be a purine such as adenine (A) and guanine (G), or a pyrimidine such as cytosine (C) and thymine (T).
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/01%3A_The_Chemical_Basis_of_Life/1.01%3A_Mixtures_and_CompoundsThis page explains the differences between mixtures and compounds, highlighting that mixtures are heterogeneous and can be separated easily, while compounds are homogeneous with fixed element proporti...This page explains the differences between mixtures and compounds, highlighting that mixtures are heterogeneous and can be separated easily, while compounds are homogeneous with fixed element proportions that do not retain individual properties. Techniques like dialysis and chromatography separate mixture components, with pure substances being elements or compounds that cannot be broken down.
- https://bio.libretexts.org/Courses/Manchester_Community_College_(MCC)/Remix_of_Openstax%3AMicrobiology_by_Parker_Schneegurt_et_al/10%3A_Modern_Applications_of_Microbial_Genetics/10.02%3A_Visualizing_and_Characterizing_DNAFinding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complemen...Finding a gene of interest within a sample requires the use of a single-stranded DNA probe labeled with a molecular beacon (typically radioactivity or fluorescence) that can hybridize with a complementary single-stranded nucleic acid in the sample. Agarose gel electrophoresis allows for the separation of DNA molecules based on size. Restriction fragment length polymorphism (RFLP) analysis allows for the visualization by agarose gel electrophoresis of distinct variants of a DNA sequence.
- https://bio.libretexts.org/Workbench/General_Biology_I_and_II/03%3A_Unit_III-_Genetics/3.04%3A_DNA_Structure_and_Function/3.4.03%3A_DNA_Structure_and_SequencingThe building blocks of DNA are nucleotides. The important components of the nucleotide are a nitrogenous base, deoxyribose (5-carbon sugar), and a phosphate group. The nucleotide is named depending on...The building blocks of DNA are nucleotides. The important components of the nucleotide are a nitrogenous base, deoxyribose (5-carbon sugar), and a phosphate group. The nucleotide is named depending on the nitrogenous base. The nitrogenous base can be a purine such as adenine (A) and guanine (G), or a pyrimidine such as cytosine (C) and thymine (T).
