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5.8: DNA Sequencing by the Dideoxy Method
https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/05%3A_DNA/5.08%3A_DNA_Sequencing_by_the_Dideoxy__Method
This page discusses the DNA sequencing process, detailing the preparation of a single strand of template DNA and the use of nucleotides and dideoxynucleotides with fluorescent tags. This method allows...This page discusses the DNA sequencing process, detailing the preparation of a single strand of template DNA and the use of nucleotides and dideoxynucleotides with fluorescent tags. This method allows for the detection of nucleotides during DNA synthesis by polymerase I. An example DNA sequence from the lysU gene of E. coli is also included, featuring 455 nucleotides.

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