This page explains the necessity of genome assembly in sequencing, due to short read lengths (45bp to 700bp) versus the large size of human genomes (3 billion bp). It describes two main strategies: cl...This page explains the necessity of genome assembly in sequencing, due to short read lengths (45bp to 700bp) versus the large size of human genomes (3 billion bp). It describes two main strategies: clone-by-clone sequencing, utilizing mapped DNA fragments, and whole genome shotgun sequencing, which assembles small fragments without a map. While shotgun sequencing is prevalent, it often needs supplementary methods for complete assemblies.
