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  • https://bio.libretexts.org/Bookshelves/Biotechnology/Introduction_to_Biotechnology_Laboratory_Manual_(Barron)/02%3A_Metrics_and_Measurements/2.02%3A_PART_I-__Metrics
    A measurement must always consist of a number and a unit, for example, 2 m, conveys the length is twice that of the base unit of length, the meter. When you are converting a smaller unit to a larger u...A measurement must always consist of a number and a unit, for example, 2 m, conveys the length is twice that of the base unit of length, the meter. When you are converting a smaller unit to a larger unit, you move the decimal point to the left the appropriate number of steps. If you want to convert 100 cm to meters (m) you would now look at your chart and determine how many “steps” you have to move the decimal to the right or left.
  • https://bio.libretexts.org/Bookshelves/Biotechnology/Introduction_to_Biotechnology_Laboratory_Manual_(Barron)/10%3A_Good_Manufacturing_Practices_(GMP)_with_New_Table
  • https://bio.libretexts.org/Bookshelves/Biotechnology/Introduction_to_Biotechnology_Laboratory_Manual_(Barron)/05%3A_Paternity_and_Gel_Electrophoresis
  • https://bio.libretexts.org/Bookshelves/Biotechnology/Introduction_to_Biotechnology_Laboratory_Manual_(Barron)/09%3A_Serial_Dilutions_and_Standard_Curve_with_a_Microplate
  • https://bio.libretexts.org/Bookshelves/Biotechnology/Introduction_to_Biotechnology_Laboratory_Manual_(Barron)/zz%3A_Back_Matter
  • https://bio.libretexts.org/Bookshelves/Biotechnology/Introduction_to_Biotechnology_Laboratory_Manual_(Barron)/01%3A_Lab_Safety_and_Lab_Notebook/1.06%3A_PUZZLE-_Lab_Safety_and_Lab_Notebooks
    hazard that the yellow color on the NFPA sign indicates rating number for the most dangerous hazards in the NFPA sign hazard that the blue color on the NFPA sign indicates the lab notebook is consider...hazard that the yellow color on the NFPA sign indicates rating number for the most dangerous hazards in the NFPA sign hazard that the blue color on the NFPA sign indicates the lab notebook is considered a ___ document and can be used in court shape of sign that lists the chemical ratings from the National Fire Protection Association hazard that the white color on the NFPA sign indicates hazard that the red color on the NFPA sign indicates
  • https://bio.libretexts.org/Bookshelves/Biotechnology/Introduction_to_Biotechnology_Laboratory_Manual_(Barron)/08%3A_Column_Chromatography_(Rev)/8.06%3A_Study_Questions
    How is protein conformation related to protein function? Did you notice if your lysed cell solution looked more or less bright than the fraction you collected from the column? If you observed a differ...How is protein conformation related to protein function? Did you notice if your lysed cell solution looked more or less bright than the fraction you collected from the column? If you observed a difference, why might that be the case? Why are you able to use the column to separate your green fluorescent protein from the other cellular components? For this procedure, what could be adjusted to increase purity of your protein in the sample?
  • https://bio.libretexts.org/Bookshelves/Biotechnology/Introduction_to_Biotechnology_Laboratory_Manual_(Barron)/08%3A_Column_Chromatography_(Rev)/8.01%3A_Student_Learning_Outcomes
    Goals Explain the conditions for bacterial growth and relate it to the goal of collecting protein Explain what is meant by protein folding Describe the relationship between a protein’s conformation (t...Goals Explain the conditions for bacterial growth and relate it to the goal of collecting protein Explain what is meant by protein folding Describe the relationship between a protein’s conformation (three-dimensional shape) and function of the protein Use column chromatography to separate proteins Student Learning Outcomes Lyse bacterial cells to release protein Purify GFP via hydrophobic interaction column Identify the location of GFP throughout the purification process
  • https://bio.libretexts.org/Bookshelves/Biotechnology/Introduction_to_Biotechnology_Laboratory_Manual_(Barron)/03%3A_Micropipetting/3.05%3A_Study_Questions
    Convert the following: 345 mL = __________________ µl 0.54 mL = _________________ µl Which micropipette would you choose to measure 550µl? Make 3 suggestions that other biotechnologists can use to imp...Convert the following: 345 mL = __________________ µl 0.54 mL = _________________ µl Which micropipette would you choose to measure 550µl? Make 3 suggestions that other biotechnologists can use to improve micropipetting accuracy. Assuming that the density of water is 1 gram per milliliter, how much should 550 uL of water weigh? 17 uL of water? 167 uL of water? What is the formula to calculate percent error? What is the maximum volume you can set for each micropipette (P-1000, P-200, P-20)?
  • https://bio.libretexts.org/Bookshelves/Biotechnology/Introduction_to_Biotechnology_Laboratory_Manual_(Barron)/01%3A_Lab_Safety_and_Lab_Notebook
  • https://bio.libretexts.org/Bookshelves/Biotechnology/Introduction_to_Biotechnology_Laboratory_Manual_(Barron)/07%3A_Transformation/7.01%3A_Student_Learning_Outcomes
    Goals Describe the role of transformation in the gene cloning process Explain the purpose of each control in the transformation experiment Explain how the information encoded in a gene is expressed as...Goals Describe the role of transformation in the gene cloning process Explain the purpose of each control in the transformation experiment Explain how the information encoded in a gene is expressed as a trait Student Learning Outcomes Carryout a transformation Predict the growth results for both negative control, plasmid containing reaction on both antibiotic-containing and nutrient agar only media Develop a reasoning, if predicted growth results don’t match actual growth results

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