The Western blot technique determines protein molecular weight and measures protein abundance in different samples.It is a widely accepted analytical technique used to detect specific proteins in a gi...The Western blot technique determines protein molecular weight and measures protein abundance in different samples.It is a widely accepted analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. Western blot samples can be taken from whole tissue or from cell culture. Solid tissues are first broken down mechanically using either a blender (for larger sample volumes), a homogenizer (smaller volumes), or by sonication.
