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- https://bio.libretexts.org/Courses/North_Central_State_College/BIOL_1550%3A_Microbiology_(2025)/02%3A_Microscopes/2.04%3A_Staining_Microscopic_SpecimensIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
- https://bio.libretexts.org/Courses/North_Carolina_State_University/MB352_General_Microbiology_Laboratory_2021_(Lee)/04%3A_Staining_Techniques/4.01%3A_Introduction_to_StainingBy dissolving these layers, the crystal violet-iodine complex is also removed, and thus Gram negatives are now able to take up the secondary stain, safranin, which is used in the last step of the Gram...By dissolving these layers, the crystal violet-iodine complex is also removed, and thus Gram negatives are now able to take up the secondary stain, safranin, which is used in the last step of the Gram stain, staining them pinkish-red and differentiating between them and the Gram positives, who with their thick peptidoglycan layer has retained the primary stain, crystal violet, and appears purple/blue.
- https://bio.libretexts.org/Courses/Portland_Community_College/Cascade_Microbiology/02%3A_How_We_See_the_Invisible_World/2.4%3A_Staining_Microscopic_SpecimensIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Microbiology/02%3A_How_We_See_the_Invisible_World/2.03%3A_Staining_Microscopic_SpecimensIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)/03%3A_Preparation_of_Bacterial_Smears_and_Introduction_to_Staining/3.01%3A_IntroductionMost types of cells do not have much natural pigment and are therefore difficult to see under the light microscope unless stained. Several types of stains are used to make bacterial cells more visible...Most types of cells do not have much natural pigment and are therefore difficult to see under the light microscope unless stained. Several types of stains are used to make bacterial cells more visible. In addition, specific staining techniques can be used to determine the cells’ biochemical or structural properties, such as cell wall type and presence or absence of endospores. This type of information can help scientists identify and classify microorganisms.
- https://bio.libretexts.org/Courses/Mansfield_University_of_Pennsylvania/BSC_3271%3A_Microbiology_for_Health_Sciences_Sp21_(Kagle)/01%3A_Introduction/1.03%3A_How_We_See_the_Invisible_World/1.3.04%3A_Staining_Microscopic_SpecimensIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
- https://bio.libretexts.org/Bookshelves/Microbiology/Microbiology_(OpenStax)/02%3A_How_We_See_the_Invisible_World/2.04%3A_Staining_Microscopic_SpecimensIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
- https://bio.libretexts.org/Courses/Manchester_Community_College_(MCC)/Remix_of_Openstax%3AMicrobiology_by_Parker_Schneegurt_et_al/03%3A_Microscope_and_the_Cell/3.02%3A_Staining_Microscopic_SpecimensIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/21%3A_Capsule_StainThe capsule is a thick polysaccharide layer around the outside of the cell. It is nonionic, so the dyes that we commonly use will not bind to it. Two dyes, one acidic and one basic, are used to stain ...The capsule is a thick polysaccharide layer around the outside of the cell. It is nonionic, so the dyes that we commonly use will not bind to it. Two dyes, one acidic and one basic, are used to stain the background and the cell wall, respectively. The area between the 2 dyes is the halo of the capsule surrounding the cell. The capsule gives added protection to the bacteria, making it virtually impossible to be phagocytosed by white blood cells.
- https://bio.libretexts.org/Courses/Concordia_University_Chicago/Microbiology_Fundamentals_Lab_Manual/06%3A_Preparation_of_Bacterial_Smears_and_Introduction_to_Staining/6.01%3A_IntroductionMost types of cells do not have much natural pigment and are therefore difficult to see under the light microscope unless stained. Several types of stains are used to make bacterial cells more visible...Most types of cells do not have much natural pigment and are therefore difficult to see under the light microscope unless stained. Several types of stains are used to make bacterial cells more visible. In addition, specific staining techniques can be used to determine the cells’ biochemical or structural properties, such as cell wall type and presence or absence of endospores. This type of information can help scientists identify and classify microorganisms.
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Microbiology_(Liu_et_al.)/02%3A_Microscopes/2.07%3A_Sample_Preparation_for_Microscopic_StainingIn their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular stru...In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. Here, we will focus on the most clinically relevant techniques developed to identify specific microbes, cellular structures, DNA sequences, or indicators of infection in tissue samples, under the microscope.
