This page describes the Ames test using transgenic Big Blue mice. These mice contain a DNA vector with elements of E. coli's lac operon. When exposed to suspected carcinogens, mutations in lacI or the...This page describes the Ames test using transgenic Big Blue mice. These mice contain a DNA vector with elements of E. coli's lac operon. When exposed to suspected carcinogens, mutations in lacI or the operator can lead to the production of beta-galactosidase. This DNA is then used to create bacteriophages that infect E. coli, resulting in clear and blue plaques; blue plaques indicate higher mutation frequency due to beta-galactosidase production.
