This page discusses ATP synthase, an enzyme complex in mitochondria responsible for ATP synthesis using proton gradients. It has two components: Fo (membrane-embedded) and F1-ATPase (in the matrix), c...This page discusses ATP synthase, an enzyme complex in mitochondria responsible for ATP synthesis using proton gradients. It has two components: Fo (membrane-embedded) and F1-ATPase (in the matrix), capable of producing over 100 ATP molecules per second. Additionally, it can convert mechanical energy into chemical energy, synthesizing ATP from ADP and Pi at a rate of about 5 ATP molecules per second through rotation, as demonstrated in research using magnetic beads and a rotating magnetic field.