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Tlr0924

Tlr0924jelly.PNG

 

 

 

 

 

 

 

 

 

 

Class: Cyanobacteriochrome

Family: DXCF Family

Origin: Thermosynechococcus elongatus

Chromophore(s): Mixed PCB/PVB

 

The Tlr0924 GAF-GGDEF fragment is perfectly functional (panels C and D, attached), so you would look at a single thing converting and would know that it is really modulating an actual output. BTW, that also makes it superior to 6012_4 + C-terminal bit because 6012 has feeble function if any in Nostoc.

 

real_figure.png

 

 

Tlr0924abs.jpg

http://larsenlab.ucdavis.edu/@api/deki/files/1582/Tlr0924.txt

 

Tlr0924photocycle.PNG

(B) Simplified four-state, four-reaction scheme for the NpF2164g3 photocycle (only isomerization, adduction formation/breaking, and primary photointermediates are shown). The first cysteine linkage of the protein to the A-ring of the PCB chromophore is not shown.

 

References

  1. Chemical Inhomogeneity in the Ultrafast Dynamics of the DXCF Cyanobacteriochrome Tlr0924, Lucy H. Freer, Peter W. Kim, Scott C. Corley, Nathan C. Rockwell, Lu Zhao, Arthur J. Thibert, J. Clark Lagarias, and Delmar S. Larsen, Journal of Physical Chemistry B, 116 (35), 10571–10581 (2012). pdf
  2. Primary and Secondary Photodynamics of the Violet/Orange Dual-Cysteine NpF2164g3 Cyanobacteriochrome Domain from Nostoc Punctiforme, Sean M. Gottlieb, Peter W. Kim, Scott C. Corley, Dorte Madsen, Samuel J. Hanke, Che-Wei Chang, Nathan C. Rockwell, Shelley S. Martin, J. Clark Lagarias, and Delmar S. Larsen, Biochemistry, 53, 1029–1040  (2014). pdf
  3. Hauck AFE, Hardman SJO, Kutta RJ, Greetham GM, Heyes DJ, Scrutton NS. (2014). The photoinitiated reaction pathway of full-length cyanobacteriochrome Tlr0924 monitored over twelve orders of magnitude. J Biol Chem, 289, 17747-17757. eScholarID:225030 | DOI:10.1074/jbc.M114.566133
  4. Hardman S, Hauck A, Clarke I, Heyes DJ, Scrutton NS. (2014). Comprehensive analysis of the green to blue photoconversion of full-length cyanobacteriochrome Tlr0924. Biophys J, 107, 2195-2203. eScholarID:234710 | DOI:10.1016/j.bpj.2014.09.020