Like S. cerevisiae, S. pombe is a model organism with its own large community of researchers. Pombase serves as the central database for information on S. pombe, functioning much like the SGD. Access Pombase at http://www.pombase.org
- Enter the name for the S. pombe homolog that you obtained in Homologene.
- Record the systematic name for your gene, which refer to the position of the gene on the chromosome. Systematic names begin with SPAC, SPBC or SPCC, corresponding to chromo- somes I, II, and III, respectively.
- Pombase stores all the information on a single page that is divided into fields. Individual fields can be expanded or minimized with an arrow by the field name. Quick links on the lower right also help with navigation.
- Navigate to the Transcipt field. The graphic will indicate whether the homolog contains an intron. The field also contains information about the exon/intron boundaries and informa- tion on 5’- and 3’-UTRs in mRNAs, when that information is available. Does the homolog to your MET gene contain introns?
- Spend a little time seeing what kind of information (e.g. gene/protein size, function, protein interactions) is available for your S. pombe ortholog. You may find Pombase to be a helpful resource when writing lab reports.