# 6: Transport and Kinetics

- Page ID
- 4534

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- 6A: Passive and Facilitated Diffusion
- This chapter will discuss diffusion processes. First, diffusion equations will be derived for cases not involving a binding receptor. Next we will derive equations for receptor-mediated diffusion across a membrane - facilitated diffusion. We will deal with the situation when the solute must be transported up a concentration gradient, a process called active transport.

- 6B: Kinetics of Simple and Enzyme-Catalyzed Reactions
- write appropriate chemical and differential equations for the rate of disappearance of reactants or appearance of products for 1st order, pseudo first order, second order, reversible first order reactions draw and interpret graphs for integrated rate equations (showing reactant or product concentrations as a function of time) and initial rate equations (showing the initial velocity vo as a function of reactant ;
- B1: Single Step Reactions
- B2. Multi-Step Reactions
- B3. Rapid Equilibrium Enzyme-Catalyzed Reactions
- B4. Steady State Enzyme-Catalyzed Reactions
- B5. Analysis of the General Michaelis-Menten Equation
- B6. More Complicated Enzyme-catalyzed Reactions
- B7. Meaning of Kinetic Constants
- B8. Experimental Determination of Kinetic Parameters

- 6C: Enzyme Inhibition
- Since structure mediates function, anything that would significantly change the structure of an enzyme would inhibit the activity of the enzyme. Hence extremes of pH and high temperature, all of which can denature the enzyme, would inhibit the enzyme in a irreversible fashion, unless it could refold properly. Alternatively we could add a small molecule which interacts noncovalently with the enzyme to either change its conformation or directly prevent substrate binding.

- 6D: More Complicated Enzymes
- In reality, many enzymes have more than one substrate (A, B) and more than one product (P, Q). For example, the enzyme alcohol dehydrogenase catalyzes the oxidation of ethanol with NAD (a biological oxidizing agent) to form acetaldehyde and NADH.