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Biology LibreTexts

Part 1: Testing for simple sugars

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  • Testing for simple sugars

    Benedict’s solution turns orange-brown in the presence of simple sugars

    Test samplesdistilled water, glucose, starch, orange juice, regular soda, diet soda, unknown

    *Shake the starch before using it.

    1.  Fill a beaker about halfway with water and bring it to a gentle boil on the hot plate

    2.  Label tubes

    3.  Add ~2 ml sample to the tube (~0.5 inch)

    4. Add ~2 ml (0.5 inch) Benedict’s solution and swirl to mix.

    Record the color in table below

    5. Put the tube in the water and heat for 3 minutes.

    6. Use test tube clamps to remove tubes from heat and record color.

    **Dispose of waste in waste beaker**

    tube sample initial color color after heat conclusion
    1 water      
    2 glucose      
    3 starch      
    4 orange juice      
    5 regular soda      
    6 diet soda      
    7 unknown