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16.4.3.5: Gibberellins

During the 1930s Japanese scientists isolated a growth-promoting substance from cultures of a fungus that parasitizes rice plants. They called it gibberellin. After the delay caused by World War II, plant physiologists in other countries succeeded in isolating more than 30 closely-related compounds. One of the most active of these - and one found as a natural hormone in the plants themselves - is gibberellic acid (GA).

 Fig. 16.4.3.5.1 GA3

GA has a number of effects on plant growth, but the most dramatic is its effect on stem growth. When applied in low concentrations to a bush or "dwarf" bean, the stem begins to grow rapidly. The length of the internodes becomes so great that the plant becomes indistinguishable from climbing or "pole" beans. GA seems to overcome the genetic limitations in many dwarf varieties.

Synthesis of gibberellins also helps grapevines climb up toward the light by causing meristems that would have developed into flowers to develop into tendrils instead.

Note

One of the 7 pairs of traits that Mendel studied in peas as he worked out the basic rules of inheritance was dwarf-tall. The recessive gene - today called le - turns out to encode an enzyme that is defective in enabling the plant to synthesize GA. The dominant gene, Le, encodes a functioning enzyme permitting normal GA synthesis and making the "tall" phenotype.

Effects of gibberellins on gene expression

Gibberellins exert their effects by altering gene transcription, the steps for which are described below.

  • Gibberellin enters the cell and binds to a soluble receptor protein called GID1 ("gibberellin-insensitive dwarf mutant 1") which now can bind to a complex of proteins (SCF) responsible for attaching ubiquitin to one or another of several DELLA proteins.
  • This triggers the destruction of the DELLA proteins by proteasomes.
  • DELLA proteins normally bind gibberellin-dependent transcription factors, a prominent one is designated PIF3/4, preventing them from binding to the DNA of control sequences of genes that are turned on by gibberellin.
  • Destruction of the DELLA proteins relieves this inhibition and gene transcription begins.

This mechanism is another of the many cases in biology where a pathway is turned on by inhibiting the inhibitor of that pathway (a double-negative is a positive).

Another example: Auxin.

Although most of the specific proteins involved are quite different, both gibberellins and auxin affect gene expression by a similar mechanism of relief of repression.

Ligand Gibberellins Auxin
Receptor GID1 TIR1
Proteasome activator SCF SCF
Transcription factor repressor DELLA Aux/IAA
Transcription factor PIF3/4 ARF1

The dwarf varieties of rice and wheat that have played such an important part in the "green revolution" carry mutant genes that

  • interfere with the synthesis of their gibberellins (in the case of rice)
  • for wheat, reduce their ability to respond to their own gibberellins (because of mutant genes for a DELLA protein).

Dwarf varieties of sorghum and more recently maize (corn) also exist, but in these cases, the mutation interferes with auxin transport, not gibberellin activity.

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