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Biology LibreTexts

9.0: Introduction

The environment of a cell is very complex, making it very diffcult, if not impossible, to study individual reactions, enzymes, or pathways within it. For this reason, biochemists prefer to isolate molecules (enzymes, DNAs, RNAs, and other molecules of interest) so they can be analyzed without interference from the millions of other processes occurring simultaneously in the cell. Many of the methods used in isolating molecules from cells involve some form of chromatography. To separate compounds from their cellular environments, one must first break open (lyse) the cells.

Contributors

Dr. Kevin Ahern and Dr. Indira Rajagopal (Oregon State University)