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6.3: Two‑dimensional gels to analyze the number and position of replication origins

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    329
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    Figure 6.6.C.Summary of the patterns of fragments containing replication intermediates in two-dimensional gels.

    Question 6.4. A restriction map is shown for a portion of a chromosome below, along with the patterns on two-dimensional gels for the replication intermediates formed by each fragment. Where are the origins and termini? Can you deduce the direction of replication fork movement?

    Question 6.5. How can you calculate the position of an origin within a DNA fragment from an asymmetric fork/bubble pattern on a 2-D gel of replicating DNA molecules?


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