5.8: DNA Sequencing by the Dideoxy Method
- Page ID
- 4747
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The DNA to be sequenced is prepared as a single strand. This template DNA is supplied with
- a mixture of all four normal (deoxy) nucleotides in ample quantities
- dATP
- dGTP
- dCTP
- dTTP
- a mixture of all four dideoxynucleotides, each present in limiting quantities and each labeled with a "tag" that fluoresces a different color:
- ddATP
- ddGTP
- ddCTP
- ddTTP
- DNA polymerase I