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Biology LibreTexts

B2. Binding of Two Identical Ligands

  • Page ID
    4913
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    Explore the interactive graphs below to see how binding of identical ligands to two sites with different affinity changes the nature of the binding graphs. The subtle changes in the graphs should make it clear that binding analyses should be done with a rigorous mathematical analysis fitting of data to the simplest hyperbolic model before moving on to more complex models.

    Hyperbolic Graph for Two Binding Sites 

    Wolfram Mathematica CDF Player - Fractional Saturation of Sites (0-1)for Binding of L to 2 Sites - Hyperbolic Plot (free plugin required)

     

    Double Reciprocal Graph for Two Binding Sites 

    Wolfram Mathematica CDF Player - Fraction Saturation of Sites (0-1) for Binding of L to 2 Sities - Double Reciprocal Plot.  (Note that 1/Y max is 1 at the y intercept)

     

    Scatchard Graph for Two Binding Sites 

    TBA

    Semilog Graph for Two Binding Sites 

    TBA