# 5B: Mathematical Analyses of Binding Graphs

Skills to Develop

- derive equations from the equation for fractional saturation Y = ([ML]/[M0] = [L]/(KD+ [L]) equations for 1/Y as a function of 1/[L] (double reciprocal plot), Y/[L] vs Y (bound/free vs bound or the Scatchard Plot) and draw a plot of Y vs log [L] (semilog plot)
- draw error bars on the plots of Y vs L, 1/Y vs I/L, Y/L vs Y and Y vs log L.
- explain from these derivative equations and graphs how to calculate determine Kd
- describe appropriate methods to fit the data from these equations with special attention given to the effect of error bars in the experimental value of Y