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Biology LibreTexts

5B: Mathematical Analyses of Binding Graphs

Skills to Develop

  • derive equations  from the equation for fractional saturation  Y = ([ML]/[M0] = [L]/(KD+ [L])  equations for 1/Y as a function of 1/[L] (double reciprocal plot), Y/[L] vs Y (bound/free vs bound or the Scatchard Plot) and draw a plot of Y vs log [L] (semilog plot)
  • draw error bars on the plots of Y vs L, 1/Y vs I/L, Y/L vs Y and Y vs log L.
  • explain from these derivative equations and graphs how to calculate determine Kd
  • describe appropriate methods to fit the data from these equations with special attention given to the effect of error bars in the experimental value of Y