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Biology LibreTexts

B. Composition, Sequence and Conformational Analysis of Proteins

Skills to Develop

describe in general terms the procedures and chemical steps in the determinations of the following for proteins: 
  • molecular weight
  • presence of certain specific amino acids
  • amino acid composition
  • N and C terminal amino acid
  • specific amino acid necessary for binding and activity
  • amino acid sequence
  • secondary structure
  • 3D structure

Thumbnail: Structure of human hemoglobin. The proteins α and β subunits are in red and blue, and the iron-containing heme groups in green. From PDB: 1GZX​. Image used with permission (GNU; Proteopedia Hemoglobin).